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| Ox-LDL对大鼠血管平滑肌细胞OPG及RANKL表达的影响 | |
| 引用本文： | 马贤骥,兰友玲,郭峻莉,李天发. Ox-LDL对大鼠血管平滑肌细胞OPG及RANKL表达的影响[J]. 海南医学院学报, 2014, 0(11): 1457-1459,1462 |
| 作者姓名： | 马贤骥 兰友玲 郭峻莉 李天发 |
| 作者单位： | 贵阳中医学院;海南医学院心血管病研究所 |
| 基金项目： | 海南省科技重点项目（ZDXM20100045） |
| 摘 要： | 目的：观察氧化低密度脂蛋白（ox-LDL）对大鼠胸主动脉血管平滑肌细胞（VSMCs）骨保护素（OPG）及核因子-κB受体活化因子配体（RANKL）表达的影响。方法：体外培养大鼠VSMCs细胞株（A7r5）,采用不同浓度Ox-LDL（75、150、300μg/mL）或不同时间（4、12、24h）处理构建VSMCs损伤模型,分别采用定量PCR、Western blot方法检测大鼠主动脉OPG、RANKL表达情况。结果：经过oxLDL干预后,VSMCs高表达OPG而低表达RANKL,且呈现浓度和时间的依赖性。结论：ox-LDL能够促进大鼠VSMCs OPG表达,并同时抑制RANKL表达。 |
| 关 键 词： | 氧化低密度脂蛋白 血管平滑肌细胞 骨保护素 核因子-κB受体活化因子配体 |
Effects of oxidized-low density lipoprotein on the expression of OPG and RANKL in rat aortic vascular smoothmuscle cells |
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| Affiliation: | MA Xian-ji , LAN You-ling, GUO Jun-li , LI Tian-fa (1. Gui yang College of Traditional Chinese Medicine, Gui yang 550000, China ; 2. Cardiovascular institute of Hainan Medical University, Haikou 570102, China) |
| Abstract: | Objective： To investigate the effects of oxidized-low density lipoprotein （ox-LDL） on the expression of osteoprotegerin （OPG） and receptor activator of NF-κB ligand （RANKL） in rat aortic vascular smooth muscle cells （VSMCs）. Methods： The cultured VSMCs （A7rS） were treated with ox-LDL by different concentration or different period of time, and OPG and RANKL expression in rat thoracic aortas were detected by qRT-PCR and Western blot analysis. Results.. Compared with control group, OPG mRNA and protein levels were increased in a dose and time-dependent manner when VSMCs were induced by ox-LDL, while RANKL mRNA and protein levels were decreased. Conclusion： These findings suggest that ox-LDL induced the expresson of OPG, and suppressed the expression of RANKL in VSMCs. |
| Keywords: | Oxidized-low density lipoprotein Vascular smooth muscle cells Osteoprotegerin Receptor activator of NF- κB ligand |
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