参附注射液对大鼠重症急性胰腺炎肾损伤的影响

目的观察参附（SF）注射液对重症急性胰腺炎（SAP）肾损伤的影响。方法将60只SD大鼠随机分为假手术组、SAP组和SF组,每组20只,SAP模型制作采用4%牛磺胆酸钠溶液逆行注射胰管内的方法,动态测定血浆BUN、Cr和TNF-α、IL-6、LPS、ATⅡ水平,观察胰腺和肾脏的病理学变化。结果制模后SAP组血浆BUN、Cr、TNF-α、IL-6、LPS、ATⅡ水平升高;SF组血浆BUN、Cr、TNF-α、IL-6、LPS、ATⅡ水平明显降低,胰腺和肾脏的病理改变明显减轻,肾功能明显改善,24h后改变更为明显。结论细胞因子、内毒素和肾素-血管紧张素系统的激活参与了SAP肾损伤的发生发展过程;参附注射液能降低免疫炎性反应和内毒素,减轻胰腺炎和肾损伤。

Effects of Shen-Fu Injection on Kidney Injury in Severe Acute Pancreatitis Rats

Objective To expore the protective effects of Shenfu injection （ SF） on kidney injury in se-vere acute pancreatitis （ SAP） rats.Methods SD rats were divided randomly into sham operation group , SAP group and SF group .Severe acute pancreatitis rat model was developed in adult male rats by retrograde injec-tion of sodium taurocholate in biliopancreatiic duct .The plasma levels of BUN,Cr and TNF-a, IL-6 , LPS, ATⅡwere measured at different time points .Morphology of kidney and pancreas was observed .Rseu lts The plasma levels of BUN,Cr and TNF-a, IL-6 , LPS, ATⅡwere elevated significantly after SAP induction .Compared with SAP group , the plasma levels of TNF-a, IL-6 , LPS and ATⅡwere gradually decreased , the renal function were improved, histological changes of pancreas and kidney were also relieved .The change was significant after 24h.Conclusion Cytokines,LPS , and activated rennin-angiotensin system might be involved in pathogenesis of the kidney injury following SAP .Shenfu injection could modulate immune function , reduce inflammatory response , and alleviate pancreatitis and kidney injury .