星点设计-效应面法优化黄芩中4种黄酮类成分的提取工艺

目的:优选黄芩中4种黄酮类成分的提取工艺,为黄芩薄层鉴别用对照提取物的研究提供参考。方法:以乙醇体积分数、溶媒用量、提取时间为自变量,黄芩苷、汉黄芩苷、黄芩素、汉黄芩素提取率的总评"归一值"为因变量,通过星点设计-效应面法优选黄芩提取工艺并进行预测分析和工艺验证。采用HPLC测定指标成分含量,流动相乙腈(A)-0.1%磷酸水(B)梯度洗脱(0~25 min,27%A;25~27 min,27%~43%A;27~40 min,43%~56%A;40~45 min,56%~85%A),检测波长274 nm。结果:最佳提取工艺为加12倍量54%乙醇回流提取2次,每次135 min。黄芩苷、汉黄芩苷、黄芩素和汉黄芩素平均提取率分别为12.53%,2.77%,0.66%,0.25%,总评"归一值"预测值和实测值偏差1.19%。结论:优选的提取工艺稳定可行、预测性良好,为黄芩资源的合理利用提供参考。

Optimization of Extraction Process of Four Flavonoids in Scutellariae Radix by Central Composite Design-response Surface Methodology

Objective: To optimize extraction process of four flavonoids in Scutellariae Radix and provide references for control extracts research of thin-layer chromatography of Scutellariae Radix. Method: Effects of ethanol concentration,solvent dosage and extracting time on overall desirability of yields of baicalin,wogonoside,baicalein and wogonin were investigated and forecasted by central composite design-response surface methodology.HPLC was adopted to determine contents of four flavonoids with mobile phase of acetonitrile-0.1% phosphoric acid solution and detection wavelength at 274 nm. Result: Optimal extraction process was as following:extracted twice with 12 times the amount of 54% ethanol for per time of 135 min.Yields of baicalin,wogonoside,baicalein and wogonin were 12.53%,2.77%,0.66%,0.25%,respectively.Bias between the predicted and observed value of overall desirability was 1.19%. Conclusion: This optimized extraction technology is stable and feasible with good prediction.