中国健康人群和甲亢患者细胞色素氧化酶CYP2C19遗传多态性的比较

目的:考察中国健康人群和甲亢患者细胞色素氧化酶CYP2C19遗传多态性的相关性.方法:应用限制性片段长度多态性分析法(PCR-RFLP)对66例甲亢患者和74例健康志愿者进行了基因多态性分析.结果:甲亢患者和健康志愿者中基因型为野生型纯合子wt/wt的分别为34.85%(23/66)和41.89(31/74);基因型为杂合子wt/m1和wt/m2的分别为50.0%(33/66)和45.94%(34/74);基因型为突变型纯合子ml/ml和ml/m2的为15.15%(10/66)和12.16%(9/74).在本实验中未发现m2/m2基因型.甲亢患者和健康志愿受试者的wt/wt和ml/m1的频率没有显著差别.结论:中国甲亢患者和健康人群细胞色素氧化酶CYP2C19遗传多态性在统计学上没有差别.

Comparison of the genotypical polymorphism of CYP2C19 in Chinese healthy and hyperthyroid populations

Objective: To study the genotypical polymorphism of CYP2C19,a cytochrome oxi-dase, in Chinese healthy and hyperthyroid populations. Methods: The study was conducted in 74 healthy and 66 hyperthyroid Chinese and their genotypical polymorphism was analyzed by RFLP (restriction fragment length polymorphism). Results: Results of analysis showed that: there was 34. 85% (23/66) homozygote of wild type (wt/wt) in hyperthyroid group versus 41.89% (31/74) in healthy group;50% (33/66) heterozygote of wt/m1 and wt/m2 in hyperthyroid group versus 45.94% (34/ 74) in healthy group and 15.15% (10/66) mutant homozygote of m1/m1 and m1/m2 in hyperthyroid group versus 12.16% (9/74) in healthy group. No m2/m2 genotype was found in the study. Conclusion : There is no statistically significant difference in genotypical polymorphism of cytochrome oxidase CYP2C19 among healthy and hyperthyroid Chinese populations.