利心冲剂对心衰大鼠血流动力学和凋亡相关基因表达的影响

目的观察利心冲剂对心衰大鼠血流动力学的影响，探讨其抑制心肌细胞凋亡的分子机制。方法50只Wistar大鼠随机分为5组：正常组、模型组、卡托普利治疗组、利心冲剂低剂量组、利心冲剂高剂量组。采取腹腔注射阿霉素（ADR，4mg／kg，每5天一次，共3次）复制大鼠心衰模型，观察心肌细胞病理学、血流动力学及凋亡相关基因Bcl—2、Bax表达的变化。结果与模型组比较，利心冲剂治疗组光镜下心肌损伤明显减轻；左室收缩压明显升高，左室舒张末压明显降低；抑制凋亡作用的Bcl-2基因表达增高、Bax／Bcl—2降低（P〈0．05）。结论利心冲剂能改善心衰大鼠心功能及抑制心肌细胞凋亡，其机制可能与上调Bcl—2基因表达及降低Bax／Bcl-2比值有关。

Effects of Lixin Granule on Relatied Genes of Apoptosis and Hemodynamic in Adriamycin - induced Heart Failure Rats

Objective To observe the effects of Lixin granule（LXG） on hemodynamic in rats with adriamycin- induced heart fail- ure,and to investigate molecular mechanisms of apoptosis. Methods Fifty Wistar rats were randomly divided as follows：Normal con- trol group, adriamycin （ADR） group, captopril -treatment group, high and low dosage LXG groups. The pathological changes of myo- cardium,hemodynamic and relative gene of apoptosis （Bcl - 2 and Bax） were observed. Results Compared with ADR group, the extent of myocardium injury significantly reduced, the levels of left ventricular pressure （LVSP） significantly increased, while left ventricular end- diastolic pressure （I.VEDP） significantly decreased, the gene expression of Bct -2 was increased and the ratio of Bax to Bcl - 2 was decreased in LXG groups （P〈0. 05）. Conclusion LXG could improve cardiac function and apoptosis of cardiomyocytes, and the mechanism might be related with up - regulating expression of Bcl - 2 gene and reducing the ratio of Bax to Bcl - 2.