Cloning and characterization of three hemolysin genes from Aeromonas salmonicida |
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| Affiliation: | 1. Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, Jilin 130062, PR China;2. Experimental Base of Agriculture, Jilin University, Changchun, Jilin 130062, PR China;1. School of Computer and Cyber Sciences, Augusta University, Georgia, USA;2. LACL, Université Paris-Est Créteil, France;1. Department of Biological Sciences, University of Alberta, Edmonton, Canada;2. Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Canada;3. Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada;1. National Pathogen Collection Center for Aquatic Animals, Key Laboratory of Aquatic Genetic Resources of the Ministry of Agriculture, Shanghai Ocean University, Shanghai, PR China;2. Aquaculture Collaborative Innovation Center of Hubei Province, PR China;1. Department of Pharmacology, Wuhan University School of Basic Medical Sciences, Wuhan, 430071, China;2. Hubei Provincial Key Laboratory for Applied Toxicology, Hubei Provincial Center for Disease Control and Prevention, Wuhan, 430079, China |
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| Abstract: | ![]()
Two hemolysin genes (ASH3 and ASH4) of Aeromonas salmonicida strain 17-2 and one (ASH1) of A. salmonicida ATCC14174 were cloned into the cosmid vector charomid 9-36 in Escherichia coli DH1. The overall amino acid sequence of the ASH3 was similar to that of the aerolysins of Aeromonas hydrophila and Aeromonas sobria, and hemolysins AHH3, AHH4, and AHH5 of A. hydrophila, and hemolysin ASA1 of A. sobria. The sequence of ASH4 was similar to that of the AHH1 hemolysin of A. hydrophila. The ASH4 hemolysin contains some homologous sequence regions of the Vibrio vulnificus and Vibrio cholerae cytolysin-hemolysin. Both ASH3 and ASH4 DNA probes reacted with all 104 strains of A. salmonicida, whereas the ASH1 DNA probe did not hybridize with any of the 104 strains studied except strain ATCC14174. ASH1 and ASH3 were broad spectrum hemolysins with most activity against rabbit and horse erythrocytes, respectively, whereas ASH4 hemolysin did not lyse bovine and horse erythrocytes. ASH3 and ASH4, but not ASH1, were activated by trypsin. |
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