环孢素对肝细胞超微结构影响的研究

目的 定量观察治疗量环孢素Ａ（ＣｓＡ）对大鼠肝脏超微结构的影响。方法 ＳＤ大鼠２４只，分为３组，每组８只。⑴对照组：口服橄榄油。⑵小剂量ＣｓＡ组：口服ＣｓＡ２５ｍｇ．ｋｇ＾－１．ｄ＾－１。⑶数密度（Ｎｖ）。⑷比表面（δ）。结果 ＣｓＡ使肝细胞内质网Ｖｖ、Ｓｖ增加，δ值降低；微体Ｖｖ、Ｎｖ降低；高尔基体Ｖｖ降低；糖原减少；脂滴Ｖｖ增加，ＣｓＡ对线粒体、溶酶体各项测定值无明显影响。结论 治疗量ＣｓＡ可

The effect of CsA on the morphology of the liver-the stereological analysis of the hepatic ultrastructure

Objective To study the effect of CsA in a therapeutic dose on the hepatic ultrastructure in rats.Methods Twenty-four SD rats were divided into 3 groups.The control group was orally administered olive oil,and the other 2 groups were orally given CsA 25 mg/kg and 50 mg/kg daily respectively.After 2 weeks.the liver samples were taken for ultrathin sections.The stereological method was used to quantify some hepatic ultrastructural parameters including volume density (Vv),surface density(Sv),numerical density(Nv) and specific surface(δ).Results CsA caused an increases of Vv and Sv and a decrease of δ in endoplasmic reticulum;a decreases of Vv and Nv in microbody;decreases of Vv in Golgi complex and glycogen inclusions;an increases of Vv and Nv in lipid inclusions.Conclusion CsA in a therapeutic dose could cause the change of hepatic ultrastructure of rat.