天津市2019年住院散发急性胃肠炎患儿中诺如病毒感染分子流行病学特征分析

目的:了解天津市2019年散发急性胃肠炎患儿中诺如病毒(Norovirus, NoV)感染的分子流行病学特征。方法:收集2019年1—12月就诊于天津市儿童医院住院散发急性胃肠炎患儿的粪便标本3 116份，收集患儿临床资料和个人信息，采用实时荧光定量PCR方法对NoV进行初筛，应用反转录PCR方法对初筛阳性标本的聚合酶...

Molecular epidemiological characteristics of norovirus in hospitalized children with sporadic acute gastroenteritis in Tianjin in 2019

Objective To investigate the molecular epidemiological characteristics of norovirus(NoV)in hospitalized children with sporadic acute gastroenteritis in Tianjin in 2019.Methods Fecal specimens and clinical data were collected from 3116 hospitalized children with sporadic acute gastroenteritis possibly caused by viral infection in Tianjin Children′Hospital between January and December,2019.Real-time quantitative PCR was used to detect NoV.Partial sequences of RNA-dependent RNA polymerase(RdRp)and capsid genes of NoV were amplified by RT-PCR.Sequence alignment and phylogenetic analysis were performed for further analysis.Results Among the 3116 specimens,809(26.0%)were positive for NoV.There were significant differences in NoV detection rate between different age groups(P=0.000),and the highest NoV detection rate(31.6%)was observed in the age group of 7-12 months.Moreover,the detection rate of NoV varied with seasons(P=0.000),and the NoV detection rate was highest in winter(39.0%).Based on the sequence analysis of RdRp and capsid genes,286 identified NoV strains belonged to six genotypes,which were GⅡ.P12-GⅡ.3,GⅡ.P16-GⅡ.2,GⅡ.P17-GⅡ.17,GⅡ.Pe-GⅡ.2,GⅡ.Pe-GⅡ.3 and GⅡ.Pe-GⅡ.4.The predominant genotype was GⅡ.Pe-GⅡ.4 Sydney 2012(61.2%),followed by GⅡ.P12-GⅡ.3(33.6%,96/286),GⅡ.Pe-GⅡ.3(2.4%,7/286),GⅡ.P16-GⅡ.2(2.1%,6/286),GⅡ.Pe-GⅡ.2(0.3%,1/286)and GⅡ.P17-GⅡ.17(0.3%,1/286).Patients carrying the NoV of GⅡ.Pe-GⅡ.4 Sydney 2012 genotype were likely to suffer from vomiting than those positive for NoV of GⅡ.P12-GⅡ.3 genotype.Conclusions NoV was an important pathogen causing acute gastroenteritis in children.GⅡ.Pe-GⅡ.4 Sydney 2012 and GⅡ.P12-GⅡ.3 were the major genotypes of NoV in hospitalized children with sporadic acute gastroenteritis in Tianjin in 2019.