IGFBP-rP1、BDNF及TrkB在子宫内膜癌中的表达及临床意义

目的探讨胰岛素样生长因子结合蛋白相关蛋白1(IGFBP-rP1)、脑源性神经营养因子(BDNF)及酪氨酸受体激酶B(TrkB)在子宫内膜癌中的表达,以及三者与子宫内膜癌临床病理特征的关系。方法选取102例子宫内膜癌患者作为研究对象,以40例正常子宫内膜组织作为对照,所有患者术前均未接受放化疗治疗;应用免疫组织化学Envison法检测IGFBP-rP1、BDNF和TrkB的阳性表达,分析三者与子宫内膜癌临床病理特征的关系,以及三者阳性表达之间的相关性。结果IGFBP-rP1、BDNF及TrkB在子宫内膜癌中的表达率分别为56.86%(58/102)、46.08%(47/102),49.02%(50/102)均高于在正常子宫内膜组织中的阳性表达率20.00%(8/40)、22.50%(9/40)、17.50%(7/40),差异均有统计学意义(χ²分别=15.74、6.69、11.93,P均<0.05);IGFBP-rP1、BDNF和TrkB的阳性表达均与内膜癌临床分期、细胞分级有关(χ²分别=4.03、4.51、5.31、5.06、6.30、7.81,P均<0.05);IGFBP-rP1在淋巴结转移组中表达率为100%(9/9),在无淋巴结转移组阳性表达率为52.69%(49/93),差异有统计学意义(χ²=3.88,P<0.05);BDNF和TrkB阳性表达率在淋巴结转移组和无淋巴结转移组中比较,差异无统计学意义(χ²分别=2.85、2.59,P均>0.05)。相关分析显示,BDNF阳性表达与TrkB阳性表达呈正相关(r=0.42,P<0.05),IGFBP-rP1阳性表达与BDNF、TrkB阳性表达均呈正相关(r分别=0.52、0.58,P均<0.05)。结论IGFBP-rP1可能通过BDNF/TrkB信号通路参与子宫内膜癌的发生发展。

Expressions and clinical significance of IGFBP-rP1,BDNF and TrkB in endometrial carcinoma

Objective To investigate the expressions of IGFBP-rP1,BDNF,TrkB in endometrial cancer and the relationship between them and the clinicopathological characteristics of endometrial cancer.Methods Totally 102 patients with endometrial cancer were selected as the study group,and 40 normal endometrial tissues were selected as control group.All patients had not receive radiotherapy and chemotherapy before surgery.Immunohistochemical with Envison System method was used to detect IGFBP-rP1,BDNF and TrkB expressions.The relationship between the three protein and the clinicopathological characteristics was analyzed,and the correlation between the positive expression of the three protein was explored.Results The positive expression rates of IGFBP-rP1,BDNF and TrkB in endometrial carcinoma were 56.86%(58/102),46.08%(47/102)and 49.02%(50/102)respectively,which were significantly higher than those in normal endometrial tissues(χ²=15.74,6.69,11.93,P<0.05).The positive expressions of IGFBP-rP1,BDNF,and TrkB were correlated with clinical stage and cell grade of endometrial carcinoma(χ²=4.03,4.51,5.31,5.06,6.30,7.81,P<0.05).The positive expression rates of IGFBP rP1 in lymphnode metastasis group and non lymphnode metastasis group were 100%(9/9)and 52.69%(49/93),the difference was statistically significant(χ²=3.88,P<0.05).The positive expression rates of BDNF and TrkB between lymphnode metastasis group and non lymphnode metastasis group was not significantly different(χ²=2.85,2.59,P>0.05).The BDNF positive expression was positively correlated with TrkB positive expression(r=0.42,P<0.05),IGFBP-rP1 positive expression was positively correlated with BDNF and TrkB positive expressions(r=0.52,0.58,P<0.05).Conclusion IGFBP-rP1 may participate in the occurrence and development of endometrial carcinoma through BDNF/TrkB signaling pathway.