双歧杆菌DNA对巨噬细胞MAPK和PKG以及NF-kB的影响

目的 以信号分子MAPK家系、PKC家族和NF-kB为线索探索青春型双歧杆菌的DNA激活巨噬细胞的途径.方法 以激光共聚焦显微镜定量测定小鼠腹腔巨噬细胞ERK1/2、JNK、p38、PKCα、PKCβⅠ、PKCβⅡ、PKCγ、PKC ε和PKCζ的含量.以细胞免疫化学方法检测巨噬细胞NF-kB的阳性细胞密度.结果 双歧杆菌DNA注射组小鼠腹腔巨噬细胞ERK1/2、PKCα和PKCβH的平均荧光强度明显高于对照组(P＜0.01),而JNK、p38、PKCβⅠ、PKCγ、PKC ε和PKCζ的平均荧光强度在两组间则差异无显著性(P＞0.05).双歧杆菌DNA注射组巨噬细胞NF-kB的阳性细胞密度显著高于对照组(P＜0.01).结论 青春型双歧杆菌的DNA可通过活化ERK1/2、PKC α、PKC βⅡ和NF-kB来激活巨噬细胞.

Influence of Bifidobacterium DNA on MAPK, PKC and NF-kB of macrophages

[Objective] To explore the pathway of DNA of Bifidobacterium adolescence activating macrophages in view of MAPK family, PKC family and NF-kB. [Methods] The contents of ERK1/2, JNK, p38, PKC α, PKC βⅠ,PKC βⅡ, PKC γ, PKCεand PKC ζ of mouse peritoneal macrophages were detected quantitatively by using laser confocal microscope. The positive cell density of NK-kB of macrophages was detected by employing immunocytochemistry method. [Results] The average fluorescent strength of ERK1/2, PKC α and PKC βⅡ produced by mouse peritoneal macrophages in Bifidobacterium DNA injection group was markedly higher than that in control group(P ＜0.01). The average fluorescent strength of JNK, p38, PKC βⅠ, PKC γ, PKC ε and PKC ζ did not have significant differences between the two groups(P ＞0.05). The positive cell density of NK-kB of macrophages in Bifidobacterium DNA injection group was markedly higher than that in control group(P ＜0.01 ). [Conclusion] DNA of bifidobacteriua adolescence could activate macrophages by promoting the activity of ERK1/2, PKC α, PKC βⅡ and NF-kB.