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| 大肠杆菌错配识别蛋白MutS的表达与活性鉴定 | |
| 引用本文： | 王芬,邢玉华,王微,谭俊杰,刘刚,张部昌,陈惠鹏. 大肠杆菌错配识别蛋白MutS的表达与活性鉴定[J]. 军事医学科学院院刊, 2011, 35(12): 917-920,927 |
| 作者姓名： | 王芬 邢玉华 王微 谭俊杰 刘刚 张部昌 陈惠鹏 |
| 作者单位： | 1. 军事医学科学院生物工程研究所,北京,100071;安徽大学生命科学学院,合肥,230039 2. 军事医学科学院生物工程研究所,北京,100071;吉林大学生命科学学院,长春,130012 3. 军事医学科学院生物工程研究所,北京,100071 4. 安徽大学生命科学学院,合肥,230039 |
| 摘 要： | 目的构建表达错配识别蛋白MutS,并进行错配识别活性鉴定。方法在大肠杆菌中表达MutS蛋白,并纯化获得目的蛋白;利用凝胶滞缓实验对错配识别活性进行验证,并计算MutS蛋白降低的错配率。结果纯化的MutS蛋白具有错配识别活性,并能降低DNA双链的错配率。结论表达纯化的MutS蛋白在体外具有特异性识别、结合含有错配碱基DNA双链的生物活性,从而有助于合成生物学的发展。 |
| 关 键 词： | 错配识别蛋白MutS 表达 纯化 活性鉴定 |
Expression and activity identification of E.coli mismatch binding protein MutS |
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| WANG Fen,XING Yu-hua,WANG Wei,TAN Jun-jie,LIU Gang,ZHANG Bu-chang,CHEN Hui-peng. Expression and activity identification of E.coli mismatch binding protein MutS[J]. Bulletin of the Academy of Military Medical Sciences, 2011, 35(12): 917-920,927 | |
| Authors: | WANG Fen XING Yu-hua WANG Wei TAN Jun-jie LIU Gang ZHANG Bu-chang CHEN Hui-peng |
| Affiliation: | WANG Fen1,2,XING Yu-hua1,3,WANG Wei1,TAN Jun-jie1,LIU Gang1,ZHANG Bu-chang2,CHEN Hui-peng1 (1.Institute of Biotechnology,Academy of Military Medical Sciences,Beijing 100071,China,2.School of Life Sciences,Anhui University,Hefei 230039,3.School of Life Sciences,Jilin University,Changchun 130012,China) |
| Abstract: | Objective To construct a vector which can successfully express mismatch recognition proteins called MutS and identify the activity of mismatch recognition.Methods MutS protein was exprssed in Escherichia coli and purified.The biological activity of the protein was identified by gel retardation assay and the mismatch rate reduced by MutS protein calculated.Results The purified MutS protein had mismatch binding activity and could reduce the mismatch rate of double-stranded DNA.Conclusion The purified MutS pro... |
| Keywords: | mismatch binding protein MutS expression purification activity identification |
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