载脂蛋白A5基因-1131T/C多态性与2型糖尿病血脂及其颈动脉内膜中层厚度相关性研究

目的 探讨载脂蛋白A5基因(ApoA5)-1131T/C多态性与成都地区汉族人群2型糖尿病(T2DM)患者血脂及其颈动脉内膜中层厚度(IMT)的关系.方法 应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)结合聚丙烯酰胺凝胶电泳(PAGE)技术,分析154例T2DM患者和206例正常对照者ApoA5基因一1131T/C多态性.酶法测定血清甘油三酯(TG)、总胆固醇(TC)及高密度脂蛋白胆固醇(HDL-C).颈动脉超声多普勒检查T2DM患者的颈动脉内膜中层厚度.结果 ApoA5基因一1131T/C多态位点T、C等位基因频率在T2DM组和正常对照组分别为0.721、0.279和0.760、0.240.基因型频率分布符合Hardy-Weinberg平衡定律.ApoA5基因-1131T/C位点基因型频率和T、C等位基因频率在T2DM组和正常对照组间比较差异无统计学意义(P>0.05).CC纯合子患T2DM风险是TT纯合子的2.432倍(95%CI:1.081～5.473).T2DM组ApoA5基因一1131T/C位点C等位基因携带者(TC)基因型和CC基因型的血清TG水平和TG/HDL-C比值均高于竹基因型携带者(P<0.05).正常对照组TC基因型携带者的血清TG水平和TG/HDL-C比值较TT基因型携带者升高(P<0.05).T2DM组颈动脉IMT ApoA5基因-1131T/C位点CC基因型亚组较TT基因型亚组有增加趋势(P=0.08).结论 ApoA5基因-1131T/C多态性与T2DM的发生无相关性,但CC基因型患T2DM的风险增加.ApDA5基因-1131T/C多态性与正常对照及T2DM患者的血清TG水平和TG/HDL-C比值增高有关,但与T2DM患者颈动脉IMT的相关性需进一步研究.

Association of Apolipoprotein A5 Gene -1131T/C Polymorphism with Serum Lipids and Carotid lntima-media Thickness in Patients with Type 2 Diabetes Mellitus

Objective To investigate the relationship between apolipoprotein A5 gene-1131T/C polymorphism and serum lipids levels and carotid intima-media thickness in patients with type 2 diabetes mellitus in a Chinese population in Chengdu.Methods The genotype and allele frequencies of apolipoprotein A5-1131T/C polymorphism were identified by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) and polyacrylamide electrophoresis(PAGE) methods.The serum lipids were measured with enzymatic kits in 154 type 2 diabetic patients and 206 normal people(control).The carotid intima-media thickness(IMT) of 116 type 2 diabetic patients was measured by B-mode ultrasonography.Results The allele frequencies of T,C in the diabetic patients were 0.721,0.279,respectively,which were not significantly different from those of the normal controls(0.760,0.240).Compared with the wild type TT,CC homozygote increased the risk of type 2 diabetes significantly(OR=2.432,95%CI:1.081-5.473).In the patients with type 2 diabetes,the serum triglyceride(TG) level and TG/HDL-C ratio were greater in those with TC and CC genotypes than those with TT genotype subjects(P<0.05).The normal people with TC genotype also had greater triglyceride levels and TG/HDL-C ratio than those with TT genotype.The diabetic patients with CC genotype had greater carotid IMT than those with TT genotype(P=0.08).Conclusion The-1131T/C polymorphism in the apolipoprotein A5 gene may have an impact on serum triglyceride levels and TG/HDL-C ratio.People with homozygote CC have increased risk of type 2 diabetes.But more evidence is needed to prove its association with carotid IMT in patients with type 2 diabetes.