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| 维生素C增加人宫颈癌Hela细胞株对顺铂敏感性的研究 | |
| 引用本文： | 李能,陈忠东. 维生素C增加人宫颈癌Hela细胞株对顺铂敏感性的研究[J]. 中国现代医学杂志, 2008, 18(12) |
| 作者姓名： | 李能 陈忠东 |
| 作者单位： | 南华大学第一附属医院,妇产科,湖南,衡阳,421001 |
| 摘 要： | 目的研究维生素C与顺铂合用对人宫颈癌Hela细胞株增殖的影响。方法用MTT比色法检测细胞生长抑制作用,流式细胞术测定各组细胞周期分布和凋亡率。结果维生素C和顺铂均可抑制Hela细胞生长,将肿瘤细胞阻滞在G0/G1期并诱导细胞凋亡。两药联合作用时表现出协同作用,细胞生长抑制率显著提高,凋亡率明显增加。结论维生素C可与顺铂协同抑制人宫颈癌Hela细胞的增殖,其协同作用的机制可能与诱导细胞凋亡有关。 |
| 关 键 词： | 维生素C 顺铂 宫颈肿瘤 Hela细胞 维生素 人宫颈癌 Hela cells 细胞株 顺铂敏感性 研究 cervical carcinoma growth cisplatin combination vitamin C 机制 细胞的增殖 协同抑制 细胞生长抑制率 协同作用 表现 联合作用 诱导细胞凋亡 细胞阻滞 |
Effect of vitamin C in combination with cisplatin on growth of cervical carcinoma Hela cells |
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| LI Neng,CHEN Zhong-dong. Effect of vitamin C in combination with cisplatin on growth of cervical carcinoma Hela cells[J]. China Journal of Modern Medicine, 2008, 18(12) | |
| Authors: | LI Neng CHEN Zhong-dong |
| Affiliation: | Department of Obstertrics and Gynecology;The First Affiliated Hospital of Nanhua University;Hengyang;Hunan 421001;P.R.China |
| Abstract: | [Objective]To study the effect of Vitamin C in combination with cisplatin on growth in human cervical cancer cell line Hela. [Methods] Cell proliferation was evaluated by MTT assay. The distribution of cell cycle,the rate of apoptosis were observed by flow cytometer respectively. [Results] Vitamin C can significantly inhibit Hela cells growth and proliferation , arrest them in G0/G1 phase and induce their apoptosis. When treatment with combination of Vitamin C and DDP, the inhibitory effect on proliferation... |
| Keywords: | vitamin C DDP cervical cancer Hela cell |
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