Identification of cis-3'-hydroxycotinine as a urinary nicotine metabolite

1. cis-3'-Hydroxycotinine was detected as an S(-)-nicotine metabolite in the urine of smokers as well as in the urine of rats and hamsters dosed with nicotine.

2. The excreted amount of cis-3'-hydroxycotinine is lower than that of the trans-isomer.