冠状动脉旁路移植术后心房颤动患者ceRNA机制的研究

目的 探讨环状RNA(circRNAs)在冠状动脉旁路移植(CABG)术后心房颤动(POAF)中的调控机制.方法 从基因表达综合数据库(GEO)的数据集基因样本表达集97455(GSE97455)中获得circRNA表达谱,使用GEO2R工具在线分析GSE97455数据集中差异表达的circRNAs(DECs),然后把...

Research on ceRNA mechanism in patients with postoperative atrial fibrillation after coronary artery bypass grafting

Objective To explore the regulatory mechanism of circular RNAs(circRNAs)in postoperative arterial fibrillation(POAF)after coronary artery bypass grafting(CABG).Methods The circRNA expression profile was obtained from data set gene sample expression 97455(GSE97455)of the Gene Expression Omnibus(GEO)database,and the differential expression of the circRNAs(DECs)in the data set GSE97455 were online analyzed employing the GEO2R software.Then the relevant probe names were transferred into international standard circRNA names;furthermore,target miRNAs and downstream mRNA of circRNAs were obtained using various bioinformatics methods.The most relevant circRNAs,miRNA and downstream target genes were screened,and the screening results were verified by RT-PCR trial.Finally,the figure of circRNA-miRNA-hub gene regulatory network was constructed and the analysis was visualized by Cytoscape 3.7.2.Results A total of 55 DECs were obtained,among which 21 circRNAs revealed up-expression and 34 circRNAs with down-expression.After deep-going analysis,the figure of circRNA-miRNA-hub gene network consisted of 6 DECs,5 miRNAs and 10 hub genes was finally obtained.Conclusion The research provides a new perspective to reveal the effect of circRNA and circRNA-miRNA-mRNA regulatory network in the POAF,and is preliminarily verified by RT-PCR trial.