HPLC法测定木瓜丸中欧前胡素的含量

目的：建立木瓜丸中欧前胡素的含量测定方法。方法：采用高效液相色谱（high performalice liquid chromatography，HPLC）法，色谱柱为Agilent Eclipse XDB—C18柱（150mm×4．6mm，5μm），流动相为甲醇-水（55：45），流速1．0ml／min，检测波长300nm。结果：欧前胡素含量在0．0364～0．1446μg范围内与峰面积线性关系良好（r=0．9998）；平均回收率为98．63％（RSD—1．02％，n=6）。结论：HPLC法灵敏、简便，结果准确，重现性好，可用于木瓜丸的质量控制。

Content Determination of Ammidin in Mugua Pill by HPLC

Objective：To develop a method for content determination of ammidin in Mugua Pill. Methods： The method of high performance liquid chromatography （HPLC） was employed. The column was Agilent Eclipse XDB-C18 （150 mm× 4.6 mm,5 μm）. The mobile phase was methanol-water（55：45）. The detection wavelength was at 300 nm. The flow rate was 1.0 ml/min. Results：There was a good linear relationship between the content of ammidin and the peak area within the range of 0. 036 4 - 0. 144 6 μg （r=0. 999 8,n=6）,The average recovery was 98.63% with RSD：1. 02% （n=6）. Conclusion：HPLC can be used for the quality control of compound Mugua Pill because of its sensitivity, convenience, accuracy and good reproducibility.