量子点荧光探针检测cNO舌鳞癌颈淋巴结微转移的研究

目的：应用特异性量子点荧光探针检测临床颈部淋巴结阴性（cN0）舌鳞癌颈部淋巴结微转移情况，比较其与免疫组化和HE染色标记转移灶的精确度。方法：用量子点荧光探针、免疫组化染色和HE染色3种方法检测39例舌鳞癌患者的586枚颈淋巴结标本，分析其微小转移灶检出率。结果：量子点荧光探针、免疫组化染色和HE染色检测颈淋巴结微转移阳性率分别是33．33％（13／39例）、25．64％（10／39例）和10．26％（4／39例），量子点免疫荧光法检出率明显高于其他两法，差异有统计学意义（P〈O．05）。结论：量子点探针荧光检测技术可应用于舌鳞癌颈部淋巴结微转移的检测。

Application of Quantum Dots Fluorescent Probes in Detecting Occult Metastasis in Cervical Lymph Nodes from cN0 Tongue Squamous Cell Carcinoma Patients.

Objective： To assess the application of quantum dot fluorescent probe in detecting occult metastasis in cervical lymph nodes from tongue squamous cell carcinoma patients. By comparing with the semi--continuous sec- tions of keratin immunohistochemistry staining technique and HE staining technique, we intend to find if quantum dot fluorescent probe is a better way to detect the occult metastasis in cervical lymph nodes of tSCC. Methods： 586 lymph nodes from 89 TSCC patients were collected and detected by optical microscopy using quantum dots fluores- cent probes and HE staining technique and semi--continuous section CK immunohistochemical staining technique respectively. Results： The detection rates of occult metastasis of the cervical lymph nodes tested by quantum dots fluorescent probes , semi--continuous sections of keratin immuno--histochemistry staining and HE staining were 33.33~ （13/39）, 25.64~/00（10/39） and 10.26%（4/39）. And the difference had statistical significance （P~0.05）. Conclusion： Quantum dot fluorescent probe, for its higher detection rates of lymph nodes metastasis, can be used in testing occult metastasis in cervical lymph nodes of tongue squamous cell carcinoma.