基因芯片技术研究虚寒证大鼠肝全基因表达谱

目的:采用基因芯片技术研究虚寒证大鼠肝全基因表达谱的改变。方法:使用中药复方生石膏、龙胆草、黄柏和知母建立虚寒证大鼠模型,应用基因芯片检测各组大鼠肝脏基因表达,筛选差异表达基因,进行基因功能分类注释。荧光定量PCR验证芯片结果。结果:虚寒模型组与空白对照组比较有99条基因差异表达,主要涉及刺激应答功能。结论:虚寒证可能通过多种刺激应答相关基因的下调,导致免疫应答功能、防御应答功能及对其他生物体刺激应答功能的降低。虚寒证的物质基础可能与此类基因的异常表达相关。

Study of Gene Chip Technology on Whole Genome Expression of Liver of Rats with Asthenia Cold Syndrome

Objective：To study the whole genome expression of liver on asthenia cold syndrome rats by gene chip technology.Methods：The Asthenia cold syndrome rats models were induced by compound preparation of traditional Chinese medicine of Raw Gypsum、Radix gentianae、Cortex Phellodendri、Anemarrhenae Rhizoma.The liver gene expression in each group was detected by gene chip.We selected the differential expression genes and conducted the significant analysis on the genetic function of differential genes.A part of genes were selected to test the accuracy of results by RT-PCR.Results：As compared to the control group,in asthenia cold model group there were 99 strips of differential expression gene,mainly about function of response to stimulus.Conclusion：Many strips of gene about response to stimulus were down-regulated in asthenia cold syndrome rats,which induced down regulation of function about immune response,defense response,response to other organism.The substance foundation of asthenia cold syndrome was possibly related to these genes.