补肾调冲方对半乳糖致POF大鼠始基卵泡募集及卵泡闭锁相关因子GDF-9表达的影响

目的:观察补肾调冲方对半乳糖致卵巢早衰(Premature ovarian failure,POF)大鼠始基卵泡募集及卵泡闭锁相关因子生长分化因子-9(Growth differentiation factor-9,GDF-9)表达的影响。方法:应用Real-time PCR法检测各组大鼠卵巢内GDF-9基因表达水平;采用Western Blot法检测各组大鼠卵巢内GDF-9蛋白表达水平。应用TUNEL法检测各组大鼠卵巢内细胞凋亡情况。结果:与空白组比较,模型组卵巢内的GDF-9 mRNA与蛋白表达水平明显减少;与模型组比较,治疗组卵巢内的GDF-9基因与蛋白表达明显增加。结论:补肾调冲方可通过调节GDF-9 mRNA与蛋白的表达,促进POF大鼠始基卵泡募集,促进生长卵泡发育,抑制卵泡异常闭锁,从而达到促进排卵,调节卵巢功能,治疗POF的目的。

Effects of Bushen Tiaochong Recipe on Galactose Toxicity-induced POF rats's Primordial Follicular Recruitment,Follicular Atresia and Associated Factor GDF-9 Expression

Objective:Study the effects of Bushen Tiaochong Recipe(BSTCR) on galactose-induced premature ovarian failure(Premature ovarian failure,POF) from primordial follicle recruitment and follicular atresia associated factor growth differentiation factor-9(Growth differentiation factor-9,GDF-9) expression.Methods:Apply Real-time PCR to detect the rat's ovarian GDF-9 gene expression,and by Western Blot to detect the rat's ovarian GDF-9 protein expression.Use TUNEL to detect the rat ovarian cell apoptosis.Results:Co...