GPIF对免疫损伤小鼠T淋巴细胞膜表面共刺激分子表达的影响

目的：分析羊胎盘免疫调节因子(GPIF)对BALB/c小鼠T淋巴细胞共刺激表面抗原分子表达及其细胞因子分泌的影响，探讨羊胎盘免疫调节因子免疫促进作用机理。方法:60Coγ-ray辐射所致免疫抑制小鼠连续7d腹腔注射GPIF，流式细胞分析术分析BALB/c小鼠脾细胞表达CD28+、CD152+单阳性细胞百分率，表达CD4+CD28+、CD8+CD28+、CD4+CD152+、CD8+CD152+双阳性细胞百分率；ELISA法检测小鼠血清IL-2、IFN-γ分泌水平。结果:羊胎盘免疫调节因子显著提高免疫损伤小鼠脾淋巴细胞CD28+、CD4+CD28+、CD8+CD28+阳性细胞百分率(P<0.05，P<0.01)，降低CD152+、CD4+CD152+阳性细胞百分率(P<0.05，P<0.01)，提高小鼠血清IL-2、IFN-γ分泌水平(P<0.01)。结论:羊胎盘免疫调节因子的免疫促进作用与其调节T淋巴细胞CD28、CD152共刺激分子通路的活化信号传递，降低T淋巴细胞的功能抑制，促进T淋巴细胞的活化有关。活化的T淋巴细胞分泌细胞因子IL-2、IFN-γ，参与细胞因子介导的免疫网络调节。

Influence of GPIF on the expression of costimulatory molecules lineaged T cells in mice with immunodeficiency in vivo

AIM:To investigate the effects of goat placenta immunoregulating factor (GPIF) on the expression of costimulatory molecules lineaged T cells in BALB/c mice. METHODS:Animal model for immunodeficiency made from BALB/c mice with whole-body irradiation by 5 Gy 60Coγ-ray was applied for research. The immunosuppressive mice were injected with GPIF for seven days continuously. FACS was applied to analyze the rate of CD28+, CD152+, CD4+CD28+, CD8+CD28+, CD4+CD152+ and CD8+CD152+ cells in splenic lymphocytes and ELISA method was employed to measure the amount of IL-2 and IFN-γ in serum of mice. RESULTS:GPIF increased the percentage of CD28+, CD4+CD28+ and CD8+CD28+ cells (P<0.05, P<0.01), and decreased the percentage of CD152+ (P<0.05, P<0.01), CD4+CD152+ cells (P<0.05, P<0.01) in splenic lymphocytes of immunosuppressive mice significantly. GPIF increased the content of IL-2 and IFN-γ in serum of mice simultaneously (P<0.01). CONCLUSION:Immuno-enhancing effect of GPIF facilitates the costimulation of CD28 pathway, which can activate T cells and accelerate the course of renewing T cell activity. The function of GPIF may have close relationship with an immune network formed by the secretion of IL-2 and IFN-γ and the expression of CD28 and CD152.