| 促甲状腺激素液相芯片检测法的建立和方法学评价 |
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| 引用本文： | 刘民,汤华,杨洋,浦永,董卓亮,李欣.促甲状腺激素液相芯片检测法的建立和方法学评价[J].上海医学检验杂志,2009(1). |
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| 作者姓名： | 刘民 汤华 杨洋 浦永 董卓亮 李欣 |
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| 作者单位： | 天津医科大学生命科学中心实验室;天津赛尔生物技术有限公司;天津市总医院特检中心; |
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| 摘 要： | 目的建立用液相芯片技术测定促甲状腺激素(TSH)的方法,对其方法学和临床应用价值作评价。方法通过抗TSH的单抗交联单色荧光微球,采用标记有生物素的另一配对抗TSH的单抗以及由藻红蛋白(PE)标记的亲合素组成相应的荧光报告系统,用液相芯片仪Luminex100检测待检标本荧光强度,通过TSH标准浓度绘制出标准TSH浓度荧光强度剂量曲线。用液相芯片技术测定100份临床血清标本的TSH浓度并与拜尔的化学发光法的测定结果做方法学比较。结果液相芯片技术测定TSH的线性范围为0.3~162μIU/mL,高、中、低3个浓度的批内精密度为10.00%、6.91%、17.80%。高、中、低3个浓度的回收率为94.3%、96.7%、95.2%。与拜尔的化学发光法的相关系数为0.965(P=8.77×10-57)。结论本研究建立的液相芯片技术在TSH测定中的主要技术指标与拜尔的化学发光法相近,有临床应用价值。
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| 关 键 词： | 液相芯片 促甲状腺激素 检测 方法学 |
Development of a liquichip assay for thyrotropic-stimulating hormone and evaluation of the methodology |
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| LIU Min,TANG Hua,YANG Yang,PU Yong,DONG Zhuoliang,LI Xin..Development of a liquichip assay for thyrotropic-stimulating hormone and evaluation of the methodology[J].Shanghai Journal of Medical Laboratory Sciences,2009(1). |
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| Authors: | LIU Min TANG Hua YANG Yang PU Yong DONG Zhuoliang LI Xin |
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| Institution: | 1.Tianjin Life Science Research Center;Tianjin Medical University;Tianjin 300070;China;2.Tianjin Saier Biotechnology Company Limited;Tianjin 300457;3.Special Laboratory Diagnosis Center;Tianjin General Hospital;Tianjin 300052;China |
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| Abstract: | Objective To develop liquichip assay for thyrotropic-stimulating hormone(TSH),and evaluate the method and the value of clinical application.Methods Monoclonal antibodies of TSH were coupled to fluorescent beads produced by Luminex corporation.Fluorescent assay system was formed by streptavidin R-phycoerythrin and the other TSH biotinylated monoclonal antibodies which were pared with the antibodies coupled to beads.Dose-responses curve was plotted with standard solutions of TSH.The Luminex100 was used to mea... |
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| Keywords: | Liquichip Thyrotropic-stimulating hormone Detection Methodology |
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