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1H NMR based profiling of spent culture media cannot predict success of implantation for day 3 human embryos
Authors:Paolo Rinaudo  Shehua Shen  Jia Hua  Su Qian  Uday Prabhu  Erwin Garcia  Marcelle Cedars  Dinesh Sukumaran  Thomas Szyperski  Chris Andrews
Affiliation:1. Department of OB GYN and Reproductive Sciences, University of California, San Francisco, CA, USA
4. Department of Clinical Research and Development, Auxogyn. Inc., Menlo Park, CA, USA
2. Departments of Chemistry and Biostatistics, State University of New York at Buffalo, Buffalo, NY, USA
5. Fresenius Kabi, Grand Island, NY, 14072, USA
3. Departments of Biostatistics, State University of New York at Buffalo, Buffalo, NY, USA
6. Bruker India, Mumbai, 400093, India
7. LipoScience Inc., 2500 Sumner Blvd., Raleigh, NC, 27616, USA
Abstract:

Background

Identification of a non-invasive technique to assess embryo implantation potential in assisted reproduction would greatly increase success rates and lead more efficiently to single embryo transfer. Early studies suggested metabonomic analysis of spent culture media could improve embryo selection. The goal of this study is to assess if embryo implantation can be predicted based on proton nuclear magnetic resonance (1H NMR) profiles of spent embryo culture media from patients undergoing transfer of multiple embryos on cycle day 3.

Method

We conducted a retrospective study in an academic assisted reproduction technology (ART) program and analyzed the data in a university research center. Two hundred twenty-eight spent culture media samples originating from 108 patients were individually analyzed. Specifically, five distinct sets (1 to 5) of different types of spent media samples (volume ~14 μL) from embryos that resulted in clinical pregnancy (positive heart rate at 6 weeks gestation) (n1 = 29; n2 = 19; n3 = 9; n4 = 12; n5 = 33; ntotal = 102) and from embryos that did not implant (n1 = 28; n2 = 29; n3 = 18; n4 = 15; n5 = 36; ntotal = 126) were collected on day 3 of embryo growth. The media samples were profiled using 1H NMR spectroscopy, and the NMR profiles of sets 1 to 5 were subject to standard uni- and multi-variate data analyses in order to evaluate potential correlation of profiles with implantation success.

Results

For set 1 of the media samples, a borderline class separation of NMR profiles was obtained by use of principal component analysis (PCA) and logistic regression. This tentative class separation could not be repeated and validated in any of the other media sets 2 to 5.

Conclusions

Despite the rigorous technical approach, 1H NMR based profiling of spent culture media cannot predict success of implantation for day 3 human embryos.

Electronic supplementary material

The online version of this article (doi:10.1007/s10815-012-9877-9) contains supplementary material, which is available to authorized users.
Keywords:ART   1H NMR   Metabonomics   IVF   Implantation success
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