A recombinant varicella vaccine harboring a respiratory syncytial virus gene induces humoral immunity |
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| Affiliation: | 1. Division of Clinical Virology, Kobe University Graduate School of Medicine, 7-5-1, Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan;2. Kanonji Institute, Seto Center, The Research Foundation for Microbial Diseases of Osaka University, 4-1-70, Seto-cho, Kanonji 768-0065, Kagawa, Japan;3. The Research Foundation for Microbial Diseases of Osaka University, 3-1, Yamada-oka, Suita 565-0871, Osaka, Japan;1. University of Zagreb, Centre for Research and Knowledge Transfer in Biotechnology, Rockefellerova 10, 10 000 Zagreb, Croatia;2. Department of Molecular and Biomedical Sciences, Jožef Stefan Institute, Jamova 39, 1000 Ljubljana, Slovenia;3. Institute of Immunology, Rockefellerova 10, 10 000 Zagreb, Croatia;4. Department of Chemistry and Biochemistry, Faculty of Chemistry and Chemical Technology, University of Ljubljana, Aškerčeva cesta 5, 1000 Ljubljana, Slovenia;5. Centre of Excellence for Integrated Approaches in Chemistry and Biology of Proteins, Jamova cesta 39, 1000 Ljubljana, Slovenia;6. Institute for Chemical Technologies and Analytics, Vienna University of Technology, Getreidemarkt 9/164, 1060 Vienna, Austria;1. Croatian Institute for Brain Research, School of Medicine, University of Zagreb, 10 000 Zagreb, Croatia;2. Faculty of Veterinary Medicine, University of Zagreb, 10 000 Zagreb, Croatia;3. New York University Abu Dhabi, United Arab Emirates;4. Université Libre de Bruxelles, 1070 Bruxelles, Belgium;1. USC Norris Comprehensive Cancer Ctr. Molecular and Computational Biology Program, Department of Biological Sciences, Departments of Pathology, Biochemistry & Molecular Biology, Molecular Microbiology & Immunology, Urology, University of Southern California Keck School of Medicine, 1441 Eastlake Ave., Rm, 5428, Los Angeles, CA 90089-9176, United States;2. Department of Physiology and Pharmacology, State University of New York Downstate Medical Center, New York, NY 11203, United States;3. Department of Microbiology and Molecular Genetics, Michigan State University, 5175 Biomedical Physical Sciences, East Lansing, MI 48824, United States |
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| Abstract: | The varicella-zoster virus (VZV) Oka vaccine strain (vOka) is highly efficient and causes few adverse events; therefore, it is used worldwide. We previously constructed recombinant vOka (rvOka) harboring the mumps virus gene. Immunizing guinea pigs with rvOka induced the production of neutralizing antibodies against the mumps virus and VZV.Here, we constructed recombinant vOka viruses containing either the respiratory syncytial virus (RSV) subgroup A fusion glycoprotein (RSV A–F) gene or RSV subgroup B fusion glycoprotein (RSV B–F) gene (rvOka-RSV A–F or rvOka-RSV B–F). Indirect immunofluorescence and Western blot analyses confirmed the expression of each recombinant RSV protein in virus-infected cells. Immunizing guinea pigs with rvOka-RSV A–F or rvOka-RSV B–F led to the induction of antibodies against RSV proteins. These results suggest that the current varicella vaccine genome can be used to generate custom-made vaccine vectors to develop the next generation of live vaccines. |
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| Keywords: | Vaccine VZV RSV Next generation vaccine BAC" },{" #name" :" keyword" ," $" :{" id" :" kw0030" }," $$" :[{" #name" :" text" ," _" :" bacterial artificial chromosome CMVp" },{" #name" :" keyword" ," $" :{" id" :" kw0040" }," $$" :[{" #name" :" text" ," _" :" cytomegalovirus promoter IFA" },{" #name" :" keyword" ," $" :{" id" :" kw0050" }," $$" :[{" #name" :" text" ," _" :" immunofluorescence analysis PFU" },{" #name" :" keyword" ," $" :{" id" :" kw0060" }," $$" :[{" #name" :" text" ," _" :" plaque-forming unit RSV" },{" #name" :" keyword" ," $" :{" id" :" kw0070" }," $$" :[{" #name" :" text" ," _" :" respiratory syncytial virus RSV G" },{" #name" :" keyword" ," $" :{" id" :" kw0080" }," $$" :[{" #name" :" text" ," _" :" RSV glycoprotein RSV F" },{" #name" :" keyword" ," $" :{" id" :" kw0090" }," $$" :[{" #name" :" text" ," _" :" RSV fusion glycoprotein vOka" },{" #name" :" keyword" ," $" :{" id" :" kw0100" }," $$" :[{" #name" :" text" ," _" :" oka vaccine strain VZVvaricella-" },{" #name" :" keyword" ," $" :{" id" :" kw0110" }," $$" :[{" #name" :" text" ," _" :" zoster virus |
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