siRNA沉默survivin基因对肺癌细胞凋亡的影响

目的观察siRNA（Small interference RNA,siRNA）介导survivin基因对肺癌细胞凋亡作用。方法设计、合成针对survivin的siRNA并构建相应载体,转染对数生长期肺癌细胞A549,对比阴性对照组和空白对照组;半定量RT-PCR检测survivin mRNA的表达,MTT法检测细胞生长,流式细胞仪检测肺癌细胞的凋亡。结果转染siRNA组survivin mRNA表达明显低于阴性对照组和空白对照组（P〈0.05）。MTT法检测各组细胞生长曲线阴性对照组与空白对照组相比,转染后24,48,96小时及1周时细胞生长未受影响,siRNA组在转染后24,48小时细胞生长未受明显影响,而96小时及一周时明显抑制。转染siRNA组的细胞的凋亡率与阴性对照组与空白对照组相比显著增加（14.94%±1.60%vs 3.23%±0.46%,4.22%±0.34%,P〈0.05）。结论本实验提示siRNA沉默survivin基因能促进肺癌细胞的凋亡,survivin siRNA基因治疗有可能成为肺癌治疗的新靶点。

Effects of survivin gene silencing by siRNA on apoptosis of lung cancer cells

Objective To investigate the effect of survivin gene silencing by siRNA on apoptosis in lung cancer.Methods SiRNAs were designed and synthesized as expression vector and transfected into lung cancer A549 cells.Compared with negative control group and blank control group,Semi-quantitive RT-PCR was used to detect survivin mRNA.Cellular growth was detected by MTT and lung cancer cell apoptosis were determined by flow cytometry.Results Expression of survivin mRNA was decreased significantly in siRNA group compared with negative control group and blank control group（P0.05）.Cellular growth at 24 h,48 h,96 h and 1wk was not affected between negative control group and blank control group.Cell growth in siRNA group was not significantly affected at 24 h and 48 h,but was sifnificantly inhibited at 96 h and 1 wk（P0.05）.In the cellular apoptic rate,the transfected group was significantly higher than those negative control group and blank control group（14.94%±1.60%vs 3.23%±0.46%,4.22%±0.34%,P0.05）.Conclusion Survivin gene silenced by siRNA might promote apoptosis of lung cancer.Survivin siRNA gene therapy would become a new target point for lung cancer.