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Isolation of Mycobacterium ulcerans from swab and fine-needle-aspiration specimens
Authors:Yeboah-Manu Dorothy  Danso Emelia  Ampah Kobina  Asante-Poku Adwoa  Nakobu Zuliehatu  Pluschke Gerd
Affiliation:1Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana;2Swiss Tropical and Public Health Institute, Basel, Switzerland;3University of Basel, Basel, Switzerland
Abstract:
For cultivation of Mycobacterium ulcerans from clinical specimens, we optimized the release of bacteria from swabs, as well as decontamination and cultivation on supplemented medium. Nevertheless, the proportions of positive cultures, 41.7% (5/12) for fine-needle-aspiration (FNA) samples and 43.8% (49/112) for swab samples, were lower than those we have previously observed for excised tissue specimens.
Keywords:
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