|
|||||
|
|
| 两株B群脑膜炎奈瑟氏菌脂寡糖减毒前后抗原性与毒性的检测 | |
| 引用本文： | 孙银燕,胡绪敬,王君. 两株B群脑膜炎奈瑟氏菌脂寡糖减毒前后抗原性与毒性的检测[J]. 中华微生物学和免疫学杂志, 1997, 0(5) |
| 作者姓名： | 孙银燕 胡绪敬 王君 |
| 作者单位： | 中国预防医学科学院流行病学微生物学研究所 |
| 摘 要： | 两株Ｂ群脑膜炎奈瑟氏菌脂寡糖减毒前后抗原性与毒性的检测孙银燕胡绪敬王君摘要脂寡糖（ＬＯＳ）是Ｂ群Ｎｍ的一种主要外膜抗原，用ＳｅｐｈａｃｒｙｌＳ－３００ＨＲ或ＳｅｐｈａｄｅｘＧ－７５凝胶层析方法从两株Ｂ群Ｎｍ（５４２８５２和３４０７）中提取获得了纯度较... |
| 关 键 词： | B群脑膜炎奈瑟氏菌 脂寡糖 寡糖 抗原性 毒性 |
Antigenicity and toxicity of LOS and OS purified from two strains of Neisseria meningitidis serogroup B |
|
| Sun Yinyan,Hu Xujing,Wang Jun. Antigenicity and toxicity of LOS and OS purified from two strains of Neisseria meningitidis serogroup B[J]. Chinese Journal of Microbiology and Immunology, 1997, 0(5) | |
| Authors: | Sun Yinyan Hu Xujing Wang Jun |
| Affiliation: | Sun Yinyan,Hu Xujing,Wang Jun. Institute of Epidemiology and Microbiology,Chinese Academy of Preventive Medicine,Beijing 102206 |
| Abstract: | Lipooligosaccharides(LOS),an important outer membrane antigenic component of Neisseria meningitidis serogroup B,were purified by gel filtration method from the strains 542852 and 3407,and were hydrolyzed by acetic acid.Then oligosaccharides(OS) were purified by Sephadex G 50 chromatography co lumn after lipid had been separated from LOS by acid hydrolyzation.Purified LOS was demonstrated to be of high antigenicity when it reacted with the sera against Nm serogroup B in ELISA or with monoclonal antibody against immunotype L3,7,9 in Dot ELISA.Although the antigenic titers of OS were lower than those of LOS,OS remained its antigenicity after LOS had been hydrolyzed by acetic acid.In addition it was demonstrated by limulus amoebocyte(LAL) test that the toxicity of OS was also reduced by 200000 to 500000 times. |
| Keywords: | Neisseria meningitidis serogroup B Lipooligosaccharide Oligosaccharide Antigenicity Toxicity |
| 本文献已被 CNKI 等数据库收录！ | |