大鼠松果体细胞增龄变化

目的:探讨大鼠松果体细胞增龄变化。方法:SD大鼠,随机分为1～2月龄组,4～5月龄组,11～12月龄组和≥24月龄组4个组。采用H-F染色、Benda胶质细胞染色和TUNEL法细胞凋亡染色光镜观察,超薄切片透射电镜观察。结果:松果体细胞数从4～5月龄组至≥24月龄组递减(P<0.001),而胶质细胞数随增龄递增(P<0.01),松果体细胞凋亡随增龄增多(P<0.01),松果体细胞胞质随增龄电子密度降低,细胞器减少,线粒体扩张、嵴断裂呈空泡状,粗面内质网排列紊乱、池扩张,脂滴增多。结论:大鼠松果体随增龄而出现松果体细胞减少、胶质细胞增多,松果体细胞退变。这些变化可能是导致机体衰老的原因之一。

Aging changes in cellular structure of pineal body in rats

Objective:To study the aging changes of the cellular structure in the pineal body in rats.Methods:Forty- eight Sprague-Dawley rats were randomly divided into four groups:1-2 months group,4-5 months group,11-12 months group and above 24 months group.Hematoxylin Eosin staining,Benda neuroglial cell staining and terminal transferase and biotin-dUTP nick end labeling(TUNEL)staining were used for light microscopic observation.The ul- trathin sections were observed by transmission electron microscopy.Results:In the 4-5 months.11-12 months and a- bove 24 months groups,the quantity of pinealocytes reduced with aging(P<0.001),but neuroglial cell increased with aging(P<0.01).The number of apoptotic cells increased with aging(P<0.01).The cytoplasm of the pine- alocytes was reduced with the decrease of electron density and the number of organdie reduced with aging.Some mito- chondria cristae broke/dissolved or disappeared;rough endoplasmic reticulum was deranged and abnormally swollen; the number of lipid droplets increased markedly.Conclusion:Structrural changes,including reduction and degeneration of the pinealocytcs and increase of the neuroglial cell with aging in pineal hody,could be one cause of the body decrepi- tude.