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| Tpc1808对H2O2诱导的C6细胞氧化应激性损伤的保护作用 | |
| 引用本文： | 顾芸,刘炎,丁斐,刘梅. Tpc1808对H2O2诱导的C6细胞氧化应激性损伤的保护作用[J]. 解剖学杂志, 2007, 30(4): 445-448,F0003 |
| 作者姓名： | 顾芸 刘炎 丁斐 刘梅 |
| 作者单位： | 南通大学江苏省神经再生重点实验室,南通,226001;南通大学江苏省神经再生重点实验室,南通,226001;南通大学江苏省神经再生重点实验室,南通,226001;南通大学江苏省神经再生重点实验室,南通,226001 |
| 基金项目： | 国家自然科学基金项目(30270427) |
| 摘 要： | 目的:研究Tpc1808对H2O2诱导的C6细胞氧化应激性损伤的保护作用。方法:采用细胞培养、基因转染、MTT、LDH、免疫荧光组织化学、Western印迹等技术,以星形胶质瘤C6细胞为研究对象,以H2O2作为氧化应激刺激物,建立细胞损伤模型。结果:当终浓度为100μmol/L的H2O2作用于C6细胞24h后,MTT减小,LDH增加,细胞出现明显凋亡;转染Tpc1808基因的细胞受到保护,grp75表达量增加,与对照组相比有显著差异。结论:Tpc1808基因可以保护H2O2引起的C6细胞氧化应激性损伤,该保护作用可能是通过增加grp75表达实现的。 |
| 关 键 词： | Tpc1808 氧化性应激 C6细胞 H2O2 |
| 修稿时间： | 2006-08-142006-11-16 |
Protective effects of Tpc1808 on rat C6 glioma cells damage induced by oxidative stress |
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| Gu Yun,Liu Yan,Ding Fei,Liu Mei. Protective effects of Tpc1808 on rat C6 glioma cells damage induced by oxidative stress[J]. Chinese Journal of Anatomy, 2007, 30(4): 445-448,F0003 | |
| Authors: | Gu Yun Liu Yan Ding Fei Liu Mei |
| Affiliation: | Jiangsu Key Laboratory of Neuroregeneration , Nantong University, Nantong 226001,China |
| Abstract: | Objective:To investigate the protective effects of Tpc1808 gene on hydrogen-peroxide(H2O2)-induced cell apoptosis in rat glioma C6 cells. Methods: MTT assay, LDH release assay and Hoechest stained were used.Results: H2O2 addition reduced the viability of C6 cells and increased the LDH release, but these were prevented by Tpc1808 pre-expressing in C6 cells. C6 cells expressing Tpc1808 significantly decreased apoptosis ratio and apoptosis bodies induced by H2O2 according to FCM analysis using PI as a fluorescent bye and Hoechst staining. Additionally, Tpc1808 induced grp75 protein expression in H2O2-induced C6 cells. Conclusion: These data indicate that Tpc1808 may inhibit ROS-mediated cytotoxic effects through induction of grp75 protein expression. |
| Keywords: | Tpc 1808 oxidative stress C6 cell H2O2 |
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