异丙酚对小鼠海马锥体神经元膜特性和突触后电流的影响

目的观察异丙酚对小鼠鼠海马锥体神经元膜特性和突触后电流的影响。方法用冰冻切片的方法将C57小鼠海马半脑切成300 μm厚度。运用全细胞膜片钳技术记录海马锥体神经元在异丙酚作用前后动作电位反应、I-V曲线及突触后电流反应后变化情况。结果异丙酚明显减少不同刺激强度下胞体动作电位产生的个数，加入异丙酚后使锥体细胞动作电位发放个数由5±3个降至2±1个（P<0.01），而对动作电位幅度无显著影响。异丙酚可改变海马锥体神经元对兴奋性电压刺激的I-V曲线平台期反应，使最大电流幅度由1647.63±124.02 pA增加至2955.08±119.10 pA（P<0.01）。异丙酚可降低锥体神经元突触后电流，加入异丙酚前为146.5±25.89 pA，加入异丙酚后为72.8±18.71 pA（P<0.01），20 min洗脱异丙酚后电流幅度恢复至132.1±30.2 pA（P<0.01）。结论异丙酚可降低海马锥体神经元动作电位发放数，改变I-V曲线兴奋性平台期反应和可逆地降低神经元突触后电流反应。

The effects of Propofol on membrane properties and postsynaptic current in hippocampal pyramidal neurons of mice

Objective To observe the effects of Propofol on membrane characteristics and postsynaptic current of hippocampal pyramidal neurons. Methods The method of frozen section cut hippocampus into 300um thickness in C57 mice. Using whole cell patch clamp technique records the hippocampal neuronal responses of action potential(AP), I-V curves and postsynaptic current during using propofol before and after. Results Propofol decreased significantly the number of action potential under different stimulus intensity before (5±3) and during (2±1) propofol application(P<0.01), but had no significant effect on the AP amplitude. Zeneca may change the hippocampal pyramidal neurons plateau reaction to excitatory voltage stimulus in I-V curve, the max current amplitude increased from（1647.63±124.02）pA to（2955.08±119.10）pA(P<0.01). Propofol can decrease the pyramidal neuronal postsynaptic current from（146.5±25.89）pA to（72.8±18.71）pA(P<0.01), and then return to（132.1± 30.2）pA (P<0.01)after washing 20 min. Conclusion Propofol can reduce hippocampal pyramidal neuronal AP number, change the I-V curve plateau responses and decreased reversibly postsynaptic currents.