SD大鼠腹部磁共振研究中呼吸麻醉的剂量控制

目的探讨在7.0T小动物磁共振研究中，呼吸麻醉剂量对实验动物和图像质量的影响及临床意义。方法选取80只130 g左右的SD大鼠，采用2.0 lps氧气加0.5%异氟烷，进行诱导麻醉，待麻醉完全后，移至检查床，按持续麻醉剂量进行分组，分为0.1%，0.15 %，0.2%，0.25%四组，每组20只，进行大鼠腹部T2 WI成像。结果80例实验动物共死亡4只，实验中进行二次麻醉15只，完成实验76只，与A、B、D组对比，C组的安全性及实用性最好，其差异有统计学意义。结论小动物腹部实验中，实验动物的呼吸质量直接影响图像的质量，实验的进程及实验结果，对病变的诊断有着直接的影响，适量的麻醉能有效控制实验动物的状态，提高实验的质量，为临床的诊断提供实验保障。

Abdominal MRI studies of SD rat breathe anesthetic of dose control

ObjectiveTo discuss the effects of breathe anesthesia on experimental and clinical significance in 7.0 T animals magnetic resonance (NMR) studies.MethodsSelected 80 SD rats, each 130 g, used oxygen (2.0 lps) plus isoflurane (0.5%) for the induction of anesthesia. After anesthesia completely, moved the rats to check bed, then devided the rats into 4 groups according to the continuous anesthesia dose (0.1%, 0.15%, 0.2%, 0.25%), with 20 rats in each group, for T2 WI imaging in rat's abdomen.ResultsIn the experiments, 4 rats were death, 15 used a second anesthesia and 76 rats completed the experiments, compared with A、B、D groups, group C was best in the security and practicability, the difference was statistically significant.ConclusionIn the experiments, the quality of breathing can directly affect the quality of the images, the experiment process and results have a direct impact to the diagnosis of lesions. Proper anesthesia dose can effectively control the status of the experimental animals, improve the quality of the experiment and provide experiment protection for clinical diagnosis.