Development of a lyophilized soybean paste certified reference material for the analysis of ochratoxin A |
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| Institution: | 1. Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 100071 PR China;2. Beijing Key Laboratory of POCT for Bioemergency and Clinic (No. BZ0329), Beijing 100071, PR China;3. Chongqing Entry Exit Inspection and Quarantine Bureau, Chongqing 400020, PR China;4. College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, PR China;5. Department of Clinical Laboratory, Chinese People''s Liberation Army General Hospital, Beijing 100853, PR China;6. Institute for Plague Prevention and Control of Hebei Province, Zhangjiakou 075000, PR China;7. School of Public Health and Management, Chongqing Medical University, Chongqing 400016, PR China;1. European Food Safety Authority (EFSA), Parma, Italy;2. Department of Food and Drug, University of Parma, Italy;3. Environmental Research and Innovation (ERIN) Department, Luxembourg Institute of Science and Technology (LIST), Belvaux, Luxembourg;4. Institute of Applied Biosciences, Karlsruhe Institute of Technology (KIT), Karlsruhe, Germany;5. Toxalim (Research Centre in Food Toxicology), Université de Toulouse, INRA, ENVT, INP-Purpan, UPS, 31027, Toulouse, France;6. UFR Sciences, Université de Bretagne Occidentale, Brest, France;7. Division of Infection Control and Environmental Health, Norwegian Institute of Public Health, Oslo, Norway |
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| Abstract: | A certified reference material (CRM) 2KRISS CRM # 108-10-018] for the analysis of ochratoxin A (OTA) in doenjang (fermented soybean paste and popular food in Korea) was produced to ensure the reliability of analytical results in testing laboratories. A home-made doenjang was chosen as a raw material after testing its OTA level. The raw material was freeze-dried, pulverized, sieved and homogenized. An isotope-dilution-liquid chromatography/tandem mass spectrometric method (ID-LC/MS/MS) which was previously developed and validated in this laboratory was used as a higher-order reference method for characterization, homogeneity studies, and short-term stability studies. The CRM had good between-bottle homogeneity with 0.56% relative standard deviation among 10 selected units. The stability of the CRM at −70 °C (the storage condition in our laboratory) and at −20 °C (the possible storage temperature at user sites) were tested for up to 8 months. No change in the OTA content was observed within the measurement uncertainty. The stability of the CRM at room temperature (for regular use and transportation) was also tested and confirmed. The certified value was (49.50 ± 1.17) μg/kg, where the expanded uncertainty was in the confidence level of 95%. |
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| Keywords: | Mycotoxin Quality control Ochratoxin A Fermented soybean paste Certified reference material Isotope dilution mass spectrometry Measurement uncertainty Homogeneity test Stability test |
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