| 人源α-synuclein跨细胞转运特性及对神经细胞突起的影响 |
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| 引用本文： | 王庆君,景玉宏,陈海超,马学珠,穆继英,高丽萍.人源α-synuclein跨细胞转运特性及对神经细胞突起的影响[J].中风与神经疾病杂志,2017(9):772-776. |
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| 作者姓名： | 王庆君 景玉宏 陈海超 马学珠 穆继英 高丽萍 |
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| 作者单位： | 1. 兰州大学基础医学院生物化学与分子生物学研究所,甘肃 兰州,730000;2. 兰州大学基础医学院人体解剖与组织胚胎学研究所,甘肃 兰州,730000 |
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| 基金项目： | 国家自然科学基金(81570725)，甘肃省中医药管理局科研课题(GZK-2014-81) |
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| 摘 要： | 目的确定人源α-synuclein是否能够跨细胞转运,过表达人源α-synuclein对神经细胞突起有何影响。方法建立稳定表达人源α-synuclein的N2a细胞株,通过接触和非接触两种方法观察人源α-synuclein的转运现象,通过测量神经突起长度,评价人源α-synuclein过表达对神经细胞突起生长的影响。结果接触和非接触两种方法均能够观察到人源α-synuclein跨细胞转运,但总体转运率低。过表达人源α-synuclein导致神经细胞突起生长不良。结论人源α-synuclein跨细胞转运现象存在,转运可能受到多因素影响,效率较低。过表达人源α-synuclein蛋白对神经突起有损伤作用。
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| 关 键 词： | 人源α-synuclein 朊蛋白样转移 细胞突起 |
Transfer of human derived α-synuclein across cell to cell and effects on neurite |
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| Abstract: | Objective To confirm whether human derived a-synuclein have ability to transfer across cell to cell .To evaluate the effect of overexpression of human derived a-synuclein on the neurite morphology .Methods Stable N2a cell lines overexpressed human derived α-synuclein were established .Contact and non-contact culture methods were used to in-vestigate the transfer of human derived α-synuclein in vitro .The length of neurite was measured during overexpression of human derived α-synuclein.Results The transfer of α-synuclein could be observed through the contact culture and non-contact culture.However,the efficiency of α-synuclein transfer was low in vitro.Additionally,high expression of human de-rived α-synuclein inhibited the neurite growth .Conclusion A little of human derived α-synuclein transfers from cell to cell in vitro.Over expression of human derived α-synuclein damages the neurite . |
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| Keywords: | Human derived α-synuclein Prion-like transfer Neurite |
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