阿魏酸对戊四氮致癫痫大鼠海马组织中Bax和Bcl-2蛋白表达的影响

目的探讨阿魏酸(Ferulic acid,FA)对戊四氮致癫痫大鼠海马组织中Bax和Bcl-2蛋白表达的影响,近而讨论FA对戊四氮致癫痫鼠海马神经元的保护作用。方法将60只雄性健康的Wister大鼠随机分为生理盐水组、模型组(PTZ 35 mg/kg)和阿魏酸干预组(50 mg/kg),均采用腹腔注射的方式,连续注射28 d,停止给药1 d后处死实验大鼠、取材。应用免疫组织化学技术和Western blot方法来检测Bax和Bcl-2的表达情况,用来判断癫痫所导致的细胞凋亡情况。结果阿魏酸干预组大鼠的海马组织Bax的表达量明显低于模型组,差异有显著性(P0.01),Bcl-2的表达量高于模型组,差异有显著性(P0.01);生理盐水组大鼠的海马组织内Bax的表达量明显低于模型组,差异有显著性(P0.01),Bcl-2表达量明显高于模型组,差异有显著性(P0.01)。结论阿魏酸对戊四氮致癫痫大鼠海马神经元损伤具有保护作用,其保护作用机制可能与其抑制鼠脑细胞凋亡有关。

Effects of ferulic acid on the expression of Bax and Bcl-2 protein in hippocampus of pentylenetetrazole-induced epi-lepsy rats

Objective To investigate the effect of ferulic acid (FA) on the expression of Bax and Bcl-2 protein in hip-pocampus of pentylenetetrazole (PTZ)-induced epilepsy rats,and to discuss the protective effect of FA on hippocampal neurons in pentylenetetrazol-induced epilepsy effect.Methods 60 male healthy wister rats were randomly divided into saline group ,model group (PTZ 35 mg/kg) and ferulic acid intervention group (50 mg/kg).The rats were injected intraperitoneally with continuous 28 days,the rats were sacrificed after one day.Immunohistochemistry and Western blot were used to detect the expression of Bax and Bcl-2,which were used to determine the apoptosis induced by epilepsy.Results The expression of Bax in the hippocampus was significantly lower than that in the model group (P<0.01).The expression of Bcl-2 was significantly higher than that of the model group (P<0.01).Conclusion Ferulic acid has protective effect on hippocampal neuronal injury induced by pentylenetet-razole in epilepsy rats,and its protective mechanism may be related to its inhibition of brain cell apoptosis .