膀胱移行细胞癌RASSF1A和Cyclin D1与Bcl-2蛋白的表达及意义

目的:探讨RASSF1A、CyclinD1和Bcl-2蛋白在膀胱移行细胞癌发生发展中的作用,以及RASSF1A和CyclinD1与Bcl-2蛋白之间的关系。方法:免疫组化法(SP法)检测45例膀胱移行细胞癌组织、45例癌旁黏膜及18例正常黏膜组织中RASSF1A、CyclinD1和Bcl-2蛋白的表达。结果:膀胱移行细胞癌组织中RASSF1A、CyclinD1和Bcl-2蛋白表达阳性率分别为46.7%(21/45)、88.9%(40/45)和57.8%(26/45);癌旁组织分别为84.4%(38/45)、42.2%(19/45)和24.4%(11/45);正常组织分别为94.4%(17/18)、38.9%(7/18)和16.7%(3/18)。膀胱移行细胞癌组织中RASSF1A蛋白的表达显著低于癌旁及正常膀胱黏膜,P<0.05;而CyclinD1和Bcl-2蛋白的表达则显著升高,P<0.05。RASSF1A和CyclinD1及Bcl-2蛋白的表达呈负相关性,P<0.05。结论:RASSF1A蛋白的表达降低和CyclinD1、Bcl-2蛋白的过表达可能参与膀胱移行细胞癌的发生发展过程,RASSF1A可能通过某种机制促使CyclinD1和Bcl-2在膀胱移行细胞癌中过表达,从而促进膀胱移行细胞癌的发生发展。

Expressions of RASSF1A,Cyclin D1,Bcl-2 and their significance in transitional cell carcinoma of bladder

OBJECTIVE:To study the expressions of RASSF1A,Cyclin D1 and Bcl-2 in transitional cell carcinoma of bladder,and study the relationship among RASSF1A protein and Cyclin D1 protein,Bcl-2 protein.METHODS:The expressions of RASSF1A protein,Cyclin D1 protein and Bcl-2 protein in transitional cell carcinoma of bladder,mucous in the paracarcinoma and normal mucous of urinary bladder,were detected by the immunohistochemical staining SP method.RESULTS:The positive expressions of RASSF1A protein,Cyclin D1 protein and Bcl-2 protein by immunohistochemistry were 46.7%(21/45),88.9%(40/45)and 57.8%(26/45)in transitional cell carcinoma of bladder,84.4%(38/45),42.2%(19/45),24.4%(11/45)in paracarcinoma mucous,94.4%(17/18),38.9%(7/18),16.7%(3/18)in normal mucous of urinary bladder,respectively.The expression of RASSF1A protein was significant lower in transitional cell carcinoma of bladder tissues than those in paracarcinoma mucous and normal mucous of urinary bladder,P<0.05.The expression of Cyclin D1 protein and Bcl-2 protein were significantly higher in transitional cell carcinoma of bladder tissues than those in paracarcinoma mucous and normal mucous of urinary bladder,P<0.05.There was a negative correlation among RASSF1A protein and Bcl-2 protein,Cyclin D1 protein in transitional cell carcinoma of bladder tissues,P<0.05.CONCLUSIONS:The abnormal expressions of RASSF1A protein,Cyclin D1 and Bcl-2 protein maybe have highly correlation with the oncogenesis and development of bladder cancer.RASSF1A protein may increase the expression of Cyclin D1 protein and Bcl-2 protein in some ways,and then lead to the oncogenesis and development of bladder cancer.