荧光定量聚合酶链反应检测HBV-DNA的意义

目的　研究HBV DNA与HBV M、乙型肝炎临床类型的相关性 (意义 )。方法　用荧光定量聚合酶联反应 (FQ PCR)检测 10 5例乙型肝炎患者HBV DNA含量 ,分析其在乙型肝炎 5项指标(HBV M)中的检出率 ,在乙型肝炎不同类型中的分布。结果　HBV DNA与HBV M对比 ,HBsAg( )、HBeAg( )、HBcAb( )患者的检出率为 97% ,HBsAg( )、HBeAb( )、HBcAb( )患者的检出率为75 % ,HBsAg( )、HBcAb( )患者的检出率为 6 0 % ,HBsAg( )患者的检出率为 4 0 % ,HBsAb( )、HBeAb( )、HBcAb( )的 (或HBsAb阳性、HBcAb阳性 )病例未检测到HBV DNA。表明HBV DNA与HBV M的抗原呈正相关 ,组间差异有非常显著意义 (P <0 0 5 )。急性乙型肝炎血清HBV DNA检出率为 72 2 % ,慢性乙型肝炎检出率为 75 % ,肝硬化检出率为 70 %。血清HBV DNA与乙型肝炎不同临床类型差异无显著意义 (P >0 0 5 )。结论　HBV DNA的复制状态与不同乙型肝炎临床类型无明显相关性。与乙型肝炎抗原呈正相关。

Significance of detecting HBV- DNA by the fluorescence quantitative PCR

Objective To study the correlativity between HBV-DNA and the markers of hepatitis B virus infection and different clinical types of hepatitis B. Methods The fluorescence quantitation (FQ) of HBV-DNA of 105 patients with hepatitis B was performed by PCR,and the correlativity between the fluorescence quantitation of HBV-DNA and the markers of hepatitis B virus and different clinical types of hepatitis B was analyzed. Results Ninety-seven percent of the patients were found HBsAg ( ),HBeAg ( ),HBcAb( );75% were HBsAg ( ),HBeAb( ),HBcAb( ) ;60% were HBsAg( ),HBcAb( );40% were HBsAg( );in HBsAb( ),HBeAb( ),HBcAb( ) (or both HBsAb and HBcAb were positive)group the HBV DNA was undetectable. The analysis indicated that there was a significant difference among different groups ( P <0.05). HBV-DNA was detected in 72.2% in acute hepatitis B group,in 75% of chronic hepatitis B group,and in 70% of cases of liver cirrhosis with hepatitis B group.The analysis indicated that there was no significant difference among the different clinical types of hepatitis( P >0.05). Conclusion The levels of viral replication were not correlated with different clinical types of hepatitis B;the concentration of HBV-DNA in serum was related to hepatitis B antigen.