人乳头瘤病毒L1壳蛋白预测宫颈上皮内瘤变患者预后的研究

目的探讨宫颈上皮内瘤变（CIN）患者治疗前人乳头瘤病毒（HPV）-L1壳蛋白的表达能否成为预测治疗后疾病转归的指标。方法通过免疫组化法检测CIN患者治疗前HPV-L1壳蛋白表达，把CIN患者分为两组：CINⅠ级组、CINⅡ~Ⅲ级组。治疗后12个月通过导流杂交基因芯片技术检测高危型人乳头瘤病毒（HR-HPV）DNA的表达。结果治疗前CINⅠ的涂片中HPV-L1壳蛋白阳性表达率为75.00％，CINⅡ~Ⅲ的涂片中HPV-L1壳蛋白阳性表达率为36.13％，两者比较差异有统计学意义（χ2=15.43，P＜0.05）。CINⅠ级组：术前HPV-L1壳蛋白阳性的患者在术后12个月随访过程中，HR-HPV持续感染阳性率为0％，而术前HPV-L1壳蛋白阴性的患者，术后HR-HPV持续感染阳性率为25.00％。两者比较差异有统计学意义（χ2=6.40，P＜0.05）；CINⅡ~Ⅲ级组：术前HPV-L1壳蛋白阳性的患者，术后12个月随访过程中，HR-HPV持续感染阳性率为2.33％；而术前HPV-L1壳蛋白阴性的患者，术后HR-HPV持续感染阳性率为22.37％。两者比较差异有统计学意义（χ2=6.44，P＜0.05）。结论 CIN患者治疗前HPV-L1壳蛋白的检测有可能成为评估CIN患者治疗后预测疾病转归的指标。

Research of human papillomavirus-L1 capsid protein for prognosing cervical intraepithelial neoplasia

Objective To investigate whether detecting human papillomavirus （HPV）-L1 capsid protein can predict the prognosis of cervical intraepithelial neoplasia （CIN） patients or not. Methods HPV-L1 capsid protein expression in pa-tient with CIN before treatment was detected through Immunocytochemical method. And then CIN patients were divided into CINⅠgroup and CINⅡ~Ⅲgroup. The expression of High-risk HPV （HR-HPV） at twelve months after treatment was detect through PCR technology. If detection of HR-HPV was positive which means HR-HPV was persistent infection. Re-sults The positive expression rate of HPV capsid protein was 75.00%in CINⅠgroup and 36.13%in CINⅡ~Ⅲgroup be-fore treatment, the difference was statistical significantly（χ2=15.43,P〈0.05）. Persistent infection rate of HR-HPV was 0%at 12 months after treatment in HPV-L1 capsid protein positive expression patients of CINⅠgroup and 25.00%in HPV-L1 capsid protein negative expression patients of CINⅠgroup, the difference was statistical significantly （χ2=6.40,P〈0.05） . Persistent infection rate of HR-HPV was 2.33%at 12 months after treatment in HPV-L1 capsid protein positive expression patients of CINⅡ~Ⅲ group and 22.37% in HPV-L1 capsid protein negative expression patients of CINⅡ~Ⅲ group, the difference was statistical significantly （χ2=6.44,P〈0.05）. Conclusion HPV-L1 capsid protein can predict the prognosis of CIN patients to some extent.