| 哮喘模型大鼠支气管平滑肌肌球蛋白轻链激酶的表达 |
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| 引用本文： | 王勇生,桂淑玉,李永怀,周青,胡向阳,吴强,汪渊.哮喘模型大鼠支气管平滑肌肌球蛋白轻链激酶的表达[J].安徽医科大学学报,2006,41(1):1-3. |
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| 作者姓名： | 王勇生 桂淑玉 李永怀 周青 胡向阳 吴强 汪渊 |
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| 作者单位： | 安徽医科大学第一附属医院呼吸内科,合肥,230032;安徽医科大学分子生物学实验室和安徽省基因研究重点实验室,合肥,230032;安徽医科大学病理学教研室,合肥,230032 |
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| 基金项目： | 安徽省教育厅自然科学基金（编号：99JL0090） |
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| 摘 要： | 目的探讨哮喘模型大鼠支气管平滑肌肌球蛋白轻链激酶（MLCK）表达的变化。方法SD大鼠24只，随机分为正常对照组（A组）、哮喘模型激发1周组（B组）和哮喘模型激发2周组（C组）。以卵蛋白（OVA）致敏和激发建立大鼠支气管哮喘模型，用免疫组织化学和免疫印迹法分析三组大鼠支气管平滑肌中MLCK表达。结果哮喘模型激发1周组大鼠支气管平滑肌MLCK表达较正常对照组明显增加，2周组增加更明显，差异均有显著性（P〈0．05）。结论支气管平滑肌MLCK表达增加可能与哮喘发病有关。
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| 关 键 词： | 哮喘 肌球蛋白轻链激酶 基因表达 大鼠 |
| 文章编号： | 1000-1492(2006)01-0001-03 |
| 收稿时间： | 2005-07-26 |
| 修稿时间： | 2005年7月26日 |
Expression of myosin light chain kinase in bronchial smooth muscle of asthma model of rat |
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| Wang Yongsheng, Gui Shuyu, Li Yonghuai, et al.Expression of myosin light chain kinase in bronchial smooth muscle of asthma model of rat[J].Acta Universitis Medicinalis Anhui,2006,41(1):1-3. |
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| Authors: | Wang Yongsheng Gui Shuyu Li Yonghuai |
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| Institution: | Dept of Respiratory Medicine, The First Affiliated Hospital of Anhui Medical University, Hefei 230022 |
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| Abstract: | Objective To investigate the expression of myosin light chain kinase ( MLCK) in bronchial smooth muscle of asthma model of rat. Methods Twenty-four SD rats were randomly divided into control group (group A), asthma model stimulated 1week group (group B) and 2 week group (group C). Asthma rat model was established by sensitization and stimulation with ovalbumin (OVA). The expressions of bronchial smooth muscle MLCK in the three groups were analysed by immunohistochemistry test and Western Blot. Results The expression of MLCK in bronchial smooth muscle of rats in group B and C was more significantly increased than that in control group(P<0.05), especially two weeks after the stimulation. Conclusion The expression increase of MLCK in bronchial smooth muscle may be correlated with asthmatic pathogenesis. |
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| Keywords: | asthma myosin-light-chain kinase gene expression rats |
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