Bcl-w在大肠癌中的表达及临床意义

目的 检测 Bcl- w 在大肠癌中的表达, 了解 Bcl- w 与临床重要病理因子的关系; 研究 Bcl- w 与Bcl- 2 基因及其 P53 基因的关系; 探讨 Bcl- w 表达对肿瘤细胞凋亡的影响。方法 免疫组化法检测 Bcl- w,Bcl- 2 及其 P53 在大肠癌中的表达; Western blot 进一步定量检测 Bcl- w 在大肠癌中的表达; TUNEL 检测大肠癌中的细胞凋亡情况。结果 全组 52 例病人中, 39 例 Bcl- w 表达阳性, 占 75%。随肿瘤浸润深度增加, 淋巴转移, 肝转移, Bcl- w 表达亦增加。3 年随访中, Bcl- w 表达阳性大肠癌患者均死亡。与 Bcl- 2 相反, Bcl- w 在大肠腺瘤中并不表达。Bcl- w 与 P53 成显著正相关, Bcl- w 与肿瘤细胞凋亡呈显著负相关。结论 Bcl- w 与大肠癌的浸润与转移密切相关。它可能是大肠癌预后相关因素之一。与 Bcl- 2 不同, Bcl- w 在肿瘤进展阶段起重要作用。此外, Bcl-w 在大肠癌细胞中起到抗凋亡作用, P53 基因下调 Bcl- w 的表达。

Bcl-w expression and its clinical significance in colorectal adenocarcinoma

Objective] To investigate Bcl-w expression and its relationship with clinical pathologic factors in colorectal carcinoma. The effect of Bcl-w expression on the apoptosis and the relationship with Bcl-2 and P53 expression were also detected in these tumors.Methods] The expression of Bel-w, Bel-2 and P53 was studied in 52 colorectal carcinoma tissue samples by using immunohistochemistry. Bel-w expression was further assessed with Western blot. The function of Bcl-w expression on the apoptosis of colorectal carcinoma was detected by TUNEL. Results] Positive immunoreactivity was identified of Bcl-w in 39 of 52(75%) colorectal cancer specimens. The expression level of Bcl-w was increased with tumor's invasion, lymph node and liver metastasis.Patients with tumor expressed Bcl-w were all died within 3 years after surgery. In contrast to Bcl-2, colorectal adenoma had not expressed Bcl-w, the statistics analysis revealed a significant negative correlation between the Bcl-w and apoptosis and a clear inverse relationship between P53 and Bcl-w expression.Conclutions] The expression of Bcl-w is significantly related to the progression of the colorectal cancer. It could be regarded as a prognostic factor for this cancer. In contrast to Bcl-2, Bcl-w play an important role in the advanced tumor stage. Furthermore, Bcl-w is a potent suppressor for apoptosis in colorectal carcinoma. An effect of P53 on down-regulating Bel-w expression is also demonstrated.