| 大鼠骨髓来源的血管内皮祖细胞的体外培养和鉴定 |
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| 引用本文： | 郑胜武,昝涛,丁力,郑清健,刘加宁,杨宇,林根辉.大鼠骨髓来源的血管内皮祖细胞的体外培养和鉴定[J].组织工程与重建外科,2010,6(5):244-247. |
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| 作者姓名： | 郑胜武 昝涛 丁力 郑清健 刘加宁 杨宇 林根辉 |
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| 作者单位： | 福建省福州市福建医科大学省立临床医学院;福建省立医院整形外科;上海交通大学医学院附属第九人民医院整复外科 |
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| 基金项目： | 福建省科技厅青年人才项目 |
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| 摘 要： | 目的探索大鼠骨髓来源的血管内皮祖细胞（EPCs）的体外培养、诱导分化及鉴定方法。方法采用密度梯度离心法从Wistar大鼠骨髓中分离出骨髓单个核细胞,并在诱导培养基（EGM-2MV）中培养,贴壁筛选法分离,诱导分化为EPCs;观察EPCs的生长分化过程,对其形态、表型、功能加以鉴定。结果培养3 d,细胞贴壁较为完全,第4天换液后,细胞呈现克隆样生长,第7～8天形成类似成熟血管内皮细胞形态,呈现典型的"铺路石"样外观;诱导培养第7、10天的贴壁细胞CD34、Flk-1及CDl33染色均呈阳性;荧光显微镜下观察,DIL-ac-LDL和FITC-UEA-1双荧光染色的细胞数占贴壁细胞数的75%以上。结论采用密度梯度离心法获得骨髓单个核细胞,在培养液中贴壁培养,可以扩增出具有典型特征的EPCs。
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| 关 键 词： | 骨髓 内皮祖细胞 体外培养 细胞鉴定 |
Culture and Identification of Rat Bone Marrow Derived-Endothelial Progenitor Cells in Vitro |
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| Authors: | ZHENG Shengwu ZAN Tao DING Li ZHENG Qingjian LIU Jianing YANG Yu LIN Genhui |
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| Institution: | 1 Department of Plastic Surgery,Fujian Provincial Hospital,Fujian Medical University,Fuzhou 350001,China;2 Department of Plastic and Reconstructive Surgery,Shanghai Ninth People′s Hospital,Shanghai Jiaotong University School of Medicine,National Tissue Engineering Center,Shanghai 200011,China. |
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| Abstract: | Objective To investigate methods of culturing,differentiation and identification of rat bone marrow derived-endothelial progenitor cells(EPCs).Methods The marrow mononuclear cells were gained from Wistar rat bone marrow by density gradient centrifugation,and cultured in microvascular endothelial growth medium-2.Cells were isolated with adherence screening method and differentiated to EPCs after induction.The procedure of EPCs growing and differentiating was observed.EPCs were identified by cellular morpholo... |
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| Keywords: | Bone marrow Endothelial progenitor cells In vitro culture cell identification |
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