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JNK/SAPK mediates doxorubicin-induced differentiation and apoptosis in MCF-7 breast cancer cells
Authors:Kim Jayoung  Freeman Michael R
Affiliation:(1) Urologic Laboratory, Department of Urology, Children's Hospital, Boston, MA, USA;(2) Department of Surgery, Harvard Medical School, Boston, MA, USA
Abstract:Pharmacologic induction of cancer cell differentiation has potential in the treatment of breast cancer. Doxorubicin, a widely used anthracycline antibiotic, was previously reported to induce differentiation of MCF-7 breast cancer cells. We demonstrate in this study that inhibition of MCF-7 breast cancer cell growth by low dose doxorubicin (0.01 µg/ml) was accompanied by an increase in cytokeratin 8/18 and milk fat globule membrane protein expression, biomarkers for differentiation of breast cancer, as well as an increase in JNK/SAPK phosphorylation. High dose doxorubicin (10.0 µg/ml) induced apoptosis in these cells. Overexpression of dominant-inhibitory forms of JNK1 and c-Jun blocked both the differentiation and apoptotic effects of doxorubicin. These results suggest that JNK/SAPK pathway signaling plays a prominent role in doxorubicin-induced cell cycle withdrawal, differentiation and control of apoptosis in this cell system. These findings support the possibility that JNK/SAPK pathway activation may be a means of therapeutic intervention in breast cancer.
Keywords:apoptosis  breast cancer  differentiation  doxorubicin  JNK/SAPK
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