| 荧光定量聚合酶链反应检测乙肝病毒及其临床意义 |
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| 摘 要： |
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| 关 键 词： | 荧光定量聚合酶链反应 检测 乙肝病毒 血清学免疫标志物 肝细胞损害 |
Quantitative detection of hepatitis B virus by fluorescence quantitative PCR and its clinical value] |
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| Authors: | Jun Chen Xian-shi Su Xuan-he Zheng |
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| Institution: | Department of Infectious Diseases, Second Xiangya Hospital, Central South University, Changsha 410011, China. |
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| Abstract: | OBJECTIVE: To study the relationship between the serum contents of HBV DNA copies and the HBV serum index of immunology and the degree of hepatocyte injury in patients with HBV hepatitis. METHODS: The contents of HBV DNA copies were detected by fluorescence quantitative PCR (FQ-PCR), and the HBV serum index of immunology by ELISA. RESULTS: The contents of HBV DNA copies had a significant difference between HBeAg (+)/anti-HBe (-) group (42 patients) and HBeAg (-)/anti-HBe (+) group (57 patients) or HBeAg (-)/anti-HBe (-) group(11 patients) (P < 0.05), while there was no significant difference between HBeAg (-)/anti-HBe (+) group and HBeAg (-)/anti-HBe (-) group(P > 0.05). The contents of HBV DNA copies had no correlation with the severe degree of hepatocyte injury(P > 0.05). CONCLUSION: Anti-HBe is not the terminal sign of HBV replication. The severe degree of hepatocyte injury has not a direct correlation with the contents of HBV DNA copies. |
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