阳离子交换树脂富集纯化益母草中总生物碱的工艺研究

［摘要］目的研究用阳离子交换树脂富集纯化益母草中总生物碱的方法和工艺条件。方法采用732阳离子交换树脂对益母草中总生物碱进行富集纯化，以分光光度法测益母草总生物碱含量为指标，考察最佳工艺和参数条件。 结果每10 g阳离子交换树脂对益母草总生物碱最大吸附量100.79 mg， 5%盐酸洗脱的解吸附率可达83.1%。经过富集纯化得益母草总生物碱提取物含量＞50%。结论732阳离子交换树脂对益母草总生物碱的吸附能力较好，可用于益母草总生物碱的提取纯化。

Study on Purification Process of Total Alkaloids from Leonurus Heterophyllus by Means of Cation Exchange Resin

Objective To study the methods and technological parameters of the purification process of total alkaloids from Leonurus Heterophyllus by means of cation exchange resin. Methods The purification process of total alkaloids from Leonurus Heterophyllus was performed by means of 732 Cation Exchange Resin , and the content of total alkaloids from Leonurus Heterophyllus was determined as an index by spectrophotometry to choose the optimal technological parameters. Results Ten grams 732 cation exchange resin could absorb 100, 79 mg of the total alkaloids ,and 5% hydrochloric acid can wash off 83.1% of the total alkaloids, The content of total alkaloids in the purified extract of Leonurus Heterophyllu was above 50%, Conclusion 732 cation exchange resin has the good ability of absorbing the total alkaloids from Leonurus Heterophyllus. It can be used for purificating total alkaloids from Leonurus Heterophyllus.