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| 病毒载体致突变性的实验研究 | |
| 引用本文： | 李贺书,李殿俊,李大林,解丽华. 病毒载体致突变性的实验研究[J]. 中国免疫学杂志, 2002, 18(9): 618-620 |
| 作者姓名： | 李贺书 李殿俊 李大林 解丽华 |
| 作者单位： | 1. 哈尔滨医科大学肿瘤研究所,哈尔滨,150040 2. 哈尔滨医科大学第三临床医学院,哈尔滨,150040 |
| 基金项目： | 黑龙江省自然科学基金 (D0 12 2 )资助 |
| 摘 要： | 目的 :观察逆转录病毒pLXSN与腺病毒LacZ作为转基因载体所构建的转基因细胞的致突变作用 ,为转基因肿瘤细胞作为瘤苗进行临床提供安全性检测参数。方法 :将病毒与细胞共培养 ,用细胞DNA通过遗传毒理学实验技术进行体内和体外的致变性实验研究。结果 :转基因细胞DNA及培养上清液未显出致突变作用。结论 :经过修饰的病毒作为转基因载体未见其致突变作用 |
| 关 键 词： | 逆转录病毒 腺病毒 肿瘤基因治疗 Ames 微核 |
| 文章编号： | 1000-484X(2002)09-0618-03 |
Study of viral vector for mutagenesis in tumor genetherapy |
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| LI He Shu,LI Dian Jun,LI Da Lin et al.Tumor Institute of Harbin Medical University,Harbin. Study of viral vector for mutagenesis in tumor genetherapy[J]. Chinese Journal of Immunology, 2002, 18(9): 618-620 | |
| Authors: | LI He Shu LI Dian Jun LI Da Lin et al.Tumor Institute of Harbin Medical University Harbin |
| Affiliation: | LI He Shu,LI Dian Jun,LI Da Lin et al.Tumor Institute of Harbin Medical University,Harbin 150040 |
| Abstract: | Objective:To observe mutagenesis of retrovirus and adenovirus as transgenic vector,and provide safe clinic evidence for transgenic tumor cell as tumor vaccin.Methods:Cells were cultured together with virus.Then,DNA and supernatant were carried on an in vestigation in mutagenesis by means of laboratory technique about genetic toxicology.Results:The result indicated that DNA and supernatant of transgenic cell had no mutagenesis through test both In vivo and in vitroConclusion:The virus modified had no mutagenesis as transgenic vector. |
| Keywords: | Retrovirus Adenovirus Cancer gene therapy Ames Micromuleus |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |