| RP-HPLC法测定百合知母汤中芒果苷、新芒果苷的含量 |
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| 引用本文： | 方前波,秦昆明,潘扬,李伟东,蔡宝昌.RP-HPLC法测定百合知母汤中芒果苷、新芒果苷的含量[J].现代中药研究与实践,2010(4):67-69. |
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| 作者姓名： | 方前波 秦昆明 潘扬 李伟东 蔡宝昌 |
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| 作者单位： | [1]国家教育部中药炮制规范化及标准化工程研究中心 南京中医药大学药学院 江苏省中药炮制重点实验室,江苏南京210029 [2]安徽中医药高等专科学校,安徽芜湖241000 |
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| 基金项目： | 国家自然科学基金重大研究计划项目（编号：90709025） |
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| 摘 要： | 目的建立一种高效液相色谱方法测定百合知母汤中芒果苷和新芒果苷的含量。方法 Agilent TC-C18色谱柱(250mm×4.6mm,5μm);流动相:乙腈和0.033%磷酸水溶液,梯度洗脱;流速:1mL·min-1;检测波长:310nm。结果芒果苷和新芒果苷分别在3.32～66.4μg·mL-1(r=0.9998)、2.58～51.6μg·mL-1(r=0.9999)范围内呈良好的线性关系。结论本方法简便、准确、可靠,可用于百合知母汤中芒果苷和新芒果苷的含量测定。
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| 关 键 词： | 百合知母汤 芒果苷 新芒果苷 含量测定 |
Determination of Mangiferin and Neomangiferin in Baihe Zhimu Decoction by RT-HPLC |
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| FANG Qian-bo,QIAN Kun-ming,PAN Yang,LI Wei-dong,CAI Bao-Chang.Determination of Mangiferin and Neomangiferin in Baihe Zhimu Decoction by RT-HPLC[J].Research and Practice on Chinese Medicines,2010(4):67-69. |
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| Authors: | FANG Qian-bo QIAN Kun-ming PAN Yang LI Wei-dong CAI Bao-Chang |
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| Institution: | 1.Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing,Nanjing University of Chinese Medicine,Jiangsu Key Laboratory of Chinese Medicine Processing,Nanjing 210029,China ;2.Anhui College of Chinese Traditional Medicine,Wuhu 241000,China) |
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| Abstract: | Objective To establish a HPLC method for the determination of mangiferin and neomangiferin in Baihe Zhimu Decoction.Methods The determination was performed on Agilent TC-C18 column（250 mm×4.6 mm,5 μm） with a grient mobile phase composed of acetionitrile and 0.033% phosphoric acid.The detection wave- length was 310 nm,and the flow rate was 1 mL·min-1.Results The linearity was obtained over the concentration range of 3.32~66.4 μg·mL-1（r=0.999 8） for mangiferin and 2.58~51.6 μg·mL-1（ r=0.999 9） for neomangiferin.Conclusion This method is smiple,accurate,reliable and is suitable for determination of mangiferin and neomangiferin. |
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| Keywords: | Baihe Zhimu decoction mangiferin neomangiferin determination |
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