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| 高效液相色谱法测定胃康胶囊中橙皮苷的含量 | |
| 引用本文： | 田玉清,王刚,赵才德. 高效液相色谱法测定胃康胶囊中橙皮苷的含量[J]. 现代中药研究与实践, 2010, 0(3): 63-64 |
| 作者姓名： | 田玉清 王刚 赵才德 |
| 作者单位： | [1]甘肃省武威市药品检验所,甘肃武威733000 [2]甘肃省武威市凉州区中医院,甘肃武威733000 |
| 摘 要： | 目的建立以高效液相色法测定胃康胶囊中橙皮苷含量的方法。方法色谱柱为CenturySIL C18-AQ(250mm×4.6mm,5μm),流动相为乙腈-0.2%磷酸溶液(21:79),流速为0.8mL·min-1,检测波长为284nm,柱温为室温,进样量为20μL。结果橙皮苷浓度在8.12～129.92μg·mL-1范围内线性关系良好,平均回收率为98.84%,RSD为1.08%。结论方法简便、准确、重现性好,可作为胃康胶囊的含量测定方法。 |
| 关 键 词： | 高效液相色谱法 胃康胶囊 橙皮苷 含量测定 |
Determination of Hesperidin in Weikang Capsules by HPLC |
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| TIAN Yu-qing,WANG Gang,ZHAO Cai-de. Determination of Hesperidin in Weikang Capsules by HPLC[J]. Research and Practice on Chinese Medicines, 2010, 0(3): 63-64 | |
| Authors: | TIAN Yu-qing WANG Gang ZHAO Cai-de |
| Affiliation: | 1.Wuwei Institute for Drug Control,Wuwei 733000,China;2.Liangzhou Traditional Chinese Medicine Hospital,Wuwei 733000,China) |
| Abstract: | Objective To establish a HPLC method for the content determination of hesperidin in Weikang capsules.Methods Chromatographic assay was performed on CenturySIL C18-AQ（250 mm×4.6 mm,5μm） column at room temperature,the mobile phase was acetonitrile-0.2%phosphoric acid solution（21：79）,the flow rate was 0.8 mL·min-1,the detection wavelength was at 284nm,the sample size was 20 μL.Results A good linear relationship were achieved when the concentration range of hesperidin was in the range of 8.12～129.92 μg·mL-1,the average recovery was 98.84% with RSD of 1.08%.Conclusion The method is simple,accurate and stable with good reproducibility,it can be used for determination of hesperidin in Weikang capsules. |
| Keywords: | HPLC Weikang capsules hesperidin content determination |
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