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| HPLC测定贯叶金丝桃滴丸中绵马贯众素含量 | |
| 引用本文： | 周鹏,曹广尚. HPLC测定贯叶金丝桃滴丸中绵马贯众素含量[J]. 中国中医药信息杂志, 2014, 0(10): 89-91 |
| 作者姓名： | 周鹏 曹广尚 |
| 作者单位： | 山东中医药大学附属医院,山东 济南,250011 |
| 基金项目： | 国家科技重大专项-重大新药创制(2009ZX09102-142)；山东省科技发展计划 |
| 摘 要： | 目的建立贯叶金丝桃滴丸中绵马贯众素含量测定的高效液相色谱方法。方法采用Kromasil-C18（150 mm×4.6 mm,5μm）色谱柱,流动相为乙腈-三氯甲烷-异丙醇-0.5%磷酸（52∶8∶30∶10）,流速1.0 mL/min,检测波长285 nm,柱温25℃。结果绵马贯众素进样量在0.236～2.124μg范围内线性关系良好（r=0.999 9）。绵马贯众素的平均加样回收率为99.64%（RSD=1.90%）。结论该方法简便、准确,适用于贯叶金丝桃滴丸中绵马贯众素的测定。 |
| 关 键 词： | 贯叶金丝桃滴丸 绵马贯众素 高效液相色谱法 |
Content Determination of Dryocrassine in Guanye Jinsitao Drop Pills by HPLC |
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| ZHOU Peng,CAO Guang-shang. Content Determination of Dryocrassine in Guanye Jinsitao Drop Pills by HPLC[J]. Chinese Journal of Information on Traditional Chinese Medicine, 2014, 0(10): 89-91 | |
| Authors: | ZHOU Peng CAO Guang-shang |
| Affiliation: | (The Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250011, China) |
| Abstract: | Objective To develop an assay of HPLC for the content determination of dryocrassine in Guanye Jinsitao drop pills. Methods The column was Kromasil-C18 （150 mm＆#215;4.6 mm, 5μm）;mobile phase was acetonitrile-chloroform-isopropanol-0.1% phosphoric acid （52∶8∶30∶10）;flow rate was 1.0 mL/min;UV detection was performed at 285 nm;detected drift tube temperature was set at 25 ℃. Results The calibration curves were linear in the range of 0.236-2.124 μg for dryocrassine （r=0.999 9）. The average recovery rate of dryocrassine was 99.64% （RSD=1.90%）. Conclusion The method is simple and accurate. It is suitable for the determination of dryocrassine in Guanye Jinsitao drop pills. |
| Keywords: | Guanye Jinsitao drop pills dryocrassine HPLC |
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