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| 尼古丁对牙胚、牙乳头间充质细胞BMP分泌影响的体外研究 | |
| 引用本文： | 王小竞,文玲英,杨富生,白玉娣,张莹,福山达郎,药师寺仁. 尼古丁对牙胚、牙乳头间充质细胞BMP分泌影响的体外研究[J]. 牙体牙髓牙周病学杂志, 2004, 14(3): 134-136 |
| 作者姓名： | 王小竞 文玲英 杨富生 白玉娣 张莹 福山达郎 药师寺仁 |
| 作者单位： | 1. 第四军医大学口腔医学院,陕西,西安,710032 2. 日本东京齿科大学小儿齿科学讲座,日本,千叶 |
| 摘 要： | 目的 :探讨尼古丁对体外鼠磨牙牙胚及牙乳头间充质细胞BMP分泌的影响。方法 :分别采用器官和细胞培养的方法体外培养牙胚和牙乳头细胞 ,在培养基和培养液中加入不同浓度的尼古丁 ,将 15d胎龄的胎鼠的下颌第一磨牙牙胚置于对照组和实验组RPMI - 16 4 0半固态培养基表面培养 4d ,人牙乳头细胞置于各组培养液中培养 3d ,之后分别制作常规组织切片和细胞爬片 ,免疫组化染色。结果 :实验组与对照组相比较 ,对照组牙胚和牙乳头细胞BMP阳性染色强于实验组 (P <0 .0 1,P <0 .0 5 )。结论 :尼古丁对体外培养胎鼠牙胚和人牙乳头细胞BMP的分泌存在抑制作用。 |
| 关 键 词： | 尼古丁 鼠牙胚 牙乳头间充质细胞 BMP 分泌 |
| 文章编号： | 1005-2593(2004)03-0134-03 |
| 修稿时间： | 2003-12-17 |
The effect of nicotine on BMP secretion of mouse molar germ and dental papilla cells of in vitro |
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| WANG Xiao-jing,WEN Ling-ying,YANG Fu-sheng,et al. The effect of nicotine on BMP secretion of mouse molar germ and dental papilla cells of in vitro[J]. Chinese Journal of Conservative Dentistry, 2004, 14(3): 134-136 | |
| Authors: | WANG Xiao-jing WEN Ling-ying YANG Fu-sheng et al |
| Abstract: | AIM:To study the effect of nicotine on BMP secretion of mouse molar dental germs and dental papilla cells in in vitro .METHODS:Add different concentration nicotine into the culture liquid and medium.First mandibular germs from 15 d old mouse embryos were seperated and cultured on semisolid medium which contains 5 g/L agar dextrose RPMI-1640 for 4 d.Dental papilla cells were cultured in different culture liquid for 3 d.Then they were made in routine histologic and climbing slice, stained with immunohistochemistry method.RESUTLS:In control group the positive expression of BMP was stronger than that of experimental group( P <0.01, P <0.05).CONCLUSION:Nicotine inhibited the BMP secretion of mouse dental germ and dental papilla mesenchyme cells in vitro . |
| Keywords: | nicotine mouse molar germ dental papilla cell BMP secretion |
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