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| 不同入药方式下三七的药效成分与砷含量测定 | |
| 引用本文： | 蒋艳雪,姜阳,朱美霖,崔斌,曹红斌,张文生. 不同入药方式下三七的药效成分与砷含量测定[J]. 中国实验方剂学杂志, 2013, 19(14): 128-131 |
| 作者姓名： | 蒋艳雪 姜阳 朱美霖 崔斌 曹红斌 张文生 |
| 作者单位： | 1. 北京师范大学,中药资源保护与利用北京市重点实验室,北京 100875;北京师范大学资源学院资源生态与中药资源研究所,北京 100875 2. 北京师范大学资源学院资源生态与中药资源研究所,北京 100875;北京师范大学,资源药物教育部工程研究中心,北京 100875 3. 北京师范大学资源学院资源生态与中药资源研究所,北京 100875;云南省三七生物技术与制药工程研究中心,昆明 650000 |
| 基金项目： | 中央高校基本科研业务费专项资金 |
| 摘 要： | 目的:通过对不同入药方式下三七中有效成分三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1和类金属砷的含量进行测定,研究不同入药方式对三七安全有效利用的影响.方法:三七生粉试样按照直接入药、水提取和乙醇提取3种方式进行前处理,经湿法消解后,采用氢化物发生原子荧光法(AFS)测定砷含量；采用高效液相色谱法(HPLC)测定有效成分含量,色谱柱为Agilent TC-C18柱(4.6 mm×250 mm,5μm),流动相为乙腈(A)-0.05％磷酸(B)进行梯度洗脱,检测波长为203 nm,流速1.0 mL·min-1,柱温30℃.结果:乙醇提取液的砷含量最少,人参皂苷Rg1、人参皂苷Rb1含量最多.结论:乙醇回流的提取方法与水煎煮的提取方法相比,其砷含量较低(平均值分别为0.023 5,0.1108 mg·kg-)且保留更多药效成分含量(总皂苷含量平均值分别为9.098％,7.738％),可能可代替水煎煮的提取方法. |
| 关 键 词： | 三七 有效成分 高效液相色谱 砷 氢化物发生原子荧光法 醇回流 |
| 收稿时间： | 2012-10-17 |
Determination of Arsenic and Pharmaceutical Components in Panax notoginseng with Different Extracting Manner |
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| JIANG Yan-xue,JIANG Yang,ZHU Mei-lin,CUI Bin,CAO Hong-bin and ZHANG Wen-sheng. Determination of Arsenic and Pharmaceutical Components in Panax notoginseng with Different Extracting Manner[J]. China Journal of Experimental Traditional Medical Formulae, 2013, 19(14): 128-131 | |
| Authors: | JIANG Yan-xue JIANG Yang ZHU Mei-lin CUI Bin CAO Hong-bin ZHANG Wen-sheng |
| Affiliation: | Beijing Area Major Laboratory of Protection and Utilization of Traditional Chinese Medicine, Beijing 100875, China;Institute of Natural Medicine and Chinese Medicine Resources, College of Resource Science and Technology, Beijing Normal University, Beijing 100875, China;Beijing Area Major Laboratory of Protection and Utilization of Traditional Chinese Medicine, Beijing 100875, China;Institute of Natural Medicine and Chinese Medicine Resources, College of Resource Science and Technology, Beijing Normal University, Beijing 100875, China;Institute of Natural Medicine and Chinese Medicine Resources, College of Resource Science and Technology, Beijing Normal University, Beijing 100875, China;Center for Natural Medicine Engineering, Ministry of Education, Beijing 100875, China;Institute of Natural Medicine and Chinese Medicine Resources, College of Resource Science and Technology, Beijing Normal University, Beijing 100875, China;Center for Natural Medicine Engineering, Ministry of Education, Beijing 100875, China;Institute of Natural Medicine and Chinese Medicine Resources, College of Resource Science and Technology, Beijing Normal University, Beijing 100875, China;Center for Natural Medicine Engineering, Ministry of Education, Beijing 100875, China;Institute of Natural Medicine and Chinese Medicine Resources, College of Resource Science and Technology, Beijing Normal University, Beijing 100875, China;Sanqi Biotechnology and Pharmaceutical Engineering Center, Kunming 650000, China |
| Abstract: | |
| Keywords: | Panax notoginseng (Burk.) F. H. Chen effective components HPLC AFS Arsenic refluxing by ethanol |
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