雪莲组织中蛋白质提取方法比较

目的:比较雪莲组织中蛋白质的提取方法.方法:采用酚法、TCA/丙酮法、尿素法提取雪莲组织中蛋白质,所得蛋白质进行一维SDS-PAGE和双向电泳检测,通过比较电泳图谱确定雪莲组织中蛋白质的最佳提取方法.结果:酚法提取的雪莲组织蛋白质杂质量最少、纯度最高,一维SDS-PAGE和双向电泳图谱显示其具有较多的蛋白质点,且蛋白质种类较多.结论:3种提取方法均有一定的蛋白质损失,建议采用酚法从雪莲组织中提取蛋白质,为雪莲蛋白质组学研究提供参考.

Comparison of Extraction Methods for Protein in Tissue of Saussurea involucrate

Objective: To comparative analyze different extraction methods for protein in tissue of Saussurea involucrate. Method: Protein in tissue of S. involucrate was extracted by phenol method,TCA/acetone method and urea method,prepared protein was detected by one-dimensional SDS-PAGE and two-dimensional electrophoresis,then optimum extraction method for protein in tissue of S. involucrate was determined by comparing electrophoretic patterns. Result: Phenol extraction method was optimal with the highest purity and the least impurities,one-dimensional SDS-PAGE and two-dimensional electrophoresis showed that there were more protein spots and species. Conclusion: Three extraction methods had some protein loss,but phenol method was the best for extracting protein from tissue of S. involucrate,this study could provide reference for investigating proteomics of S. involucrate.