鸡血藤药材及其不同部位缩合鞣质的含量测定

目的： 建立鸡血藤药材中缩合鞣质的含量测定方法，并对不同批次鸡血藤药材及其不同部位中缩合鞣质的含量进行测定。方法： 优化并建立了采用香草醛-硫酸显色体系测定鸡血藤药材中缩合鞣质含量的方法，反应条件为3 mL 3%香草醛，3 mL 40%浓硫酸，常温反应20 min，测定波长为500 nm。结果： 5个批次鸡血藤药材中缩合鞣质含量为3.99%~8.63%；其中3个批次药材不同部位的缩合鞣质含量测定结果显示，树脂状分泌物中含量最高，为8.14%~11.51%，木质部（5.39%~7.92%）和韧皮部（3.65%~6.90%）含量相对较少。结论： 香草醛-硫酸显色法快速、简便、准确、重复性好， 可用于测定鸡血藤药材中缩合鞣质的含量；缩合鞣质在树脂状分泌物中的含量比其他部位高，为传统的鸡血藤药材质量的判断方法提供了科学依据。

Determination of Condensed Tannins in Spatholobi Caulis

Objective: To establish a method for determination of condensed tannins in Spatholobi Caulis (the stem of Suberect spatholobus) and investigate the content of condensed tannins in Spatholobi Caulis and its different parts. Method: The vanillin-sulphuric acid coloration system for the determination of condensed tannins was set up. The optimized condition of coloration was 3 mL 3% vanillin, 3 mL 40% sulphuric acid, at room temperature for 20 min, and the detection wavelength was set at 500 nm. Result: The content of condensed tannins was measured as 3.99%-8.63% in five commercial crude drugs of Spatholobi Caulis. Among different parts of the crude drugs, condensed tannins was most abundant in the resin-like secretion part (8.14%-11.51%), far higher than those in the xylem part (5.39%-7.92%) and the phloem part (3.65%-6.90%). Conclusion: The developed methods are simple, rapid, accurate, stable, and reproducible with high selectivity, and are suitable for the determination of condensed tannins in the quality control of Spatholobi Caulis.